Kinetic properties of mutant human thymidine kinase 2 suggest a mechanism for mitochondrial DNA depletion myopathy

Wang, L.; Saada, A.; Eriksson, S.; J. Biol. Chem. 278, 6963-6968 (2003)

Data extracted from this reference:

EC Number

Inhibitors

Commentary

Organism

Structure

2.7.1.21

dCTP

-

Homo sapiens

2.7.1.21

deoxcytidine

-

Homo sapiens

2.7.1.21

dTTP

-

Homo sapiens

2.7.1.21

thymidine

-

Homo sapiens

EC Number

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

2.7.1.21

0.0057

-

thymidine

H121N mutant, pH 7.6

Homo sapiens

2.7.1.21

0.011

-

deoxycytidine

wild-type and H121N mutant, pH 7.6

Homo sapiens

2.7.1.21

0.013

-

thymidine

wild-type, pH 7.6

Homo sapiens

2.7.1.21

0.018

-

thymidine

I212 mutant, pH 7.6

Homo sapiens

2.7.1.21

0.025

-

ATP

wild-type, thymidine as substrate, pH 7.6

Homo sapiens

2.7.1.21

0.037

-

ATP

wild-type, deoxycytidine as substrate, pH 7.6

Homo sapiens

2.7.1.21

0.059

-

ATP

I212 mutant, deoxycytidine as substrate, pH 7.6

Homo sapiens

2.7.1.21

0.08

-

ATP

H121N mutant, thymidine as substrate, pH 7.6

Homo sapiens

2.7.1.21

0.095

-

ATP

I212 mutant, thymidine as substrate, pH 7.6

Homo sapiens

2.7.1.21

0.162

-

ATP

H121N mutant, deoxycytidine as substrate, pH 7.6

Homo sapiens

2.7.1.21

0.568

-

deoxycytidine

I212 mutant, pH 7.6

Homo sapiens

EC Number

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2.7.1.21

ATP + 2'-deoxycytidine

-

641150

Homo sapiens

ADP + 2'-deoxycytidine 5'-phosphate

-

-

-

-

EC Number

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

2.7.1.21

0.00036

-

dCTP

H121N mutant, pH 7.6, competitive with thymidine as substrate

Homo sapiens

2.7.1.21

0.00079

-

dCTP

wild-type enzyme, pH 7.6, competitive with thymidine as substrate

Homo sapiens

2.7.1.21

0.00087

-

dCTP

wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate

Homo sapiens

2.7.1.21

0.00089

-

dCTP

H121N mutant, pH 7.6, competitive with deoxycytidine as substrate

Homo sapiens

2.7.1.21

0.00089

-

dTTP

H121N mutant, pH 7.6, competitive with thymidine as substrate

Homo sapiens

2.7.1.21

0.002

-

dTTP

wild-type enzyme, pH 7.6, competitive with thymidine as substrate

Homo sapiens

2.7.1.21

0.0025

-

dTTP

wild-type and H121N mutant, pH 7.6, non-competitive with deoxycytidine as substrate

Homo sapiens

2.7.1.21

0.0036

-

thymidine

H121N mutant, pH 7.6, competitive with deoxycytidine as substrate

Homo sapiens

2.7.1.21

0.004

-

deoxycytidine

H121N mutant, pH 7.6, non-competitive with thymidine as substrate

Homo sapiens

2.7.1.21

0.0049

-

thymidine

wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate

Homo sapiens

2.7.1.21

0.04

-

deoxycytidine

wild-type enzyme, pH 7.6, non-competitive with thymidine as substrate

Homo sapiens

EC Number

Inhibitors

Commentary

Organism

Structure

2.7.1.21

dCTP

-

Homo sapiens

2.7.1.21

deoxcytidine

-

Homo sapiens

2.7.1.21

dTTP

-

Homo sapiens

2.7.1.21

thymidine

-

Homo sapiens

EC Number

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

2.7.1.21

0.00036

-

dCTP

H121N mutant, pH 7.6, competitive with thymidine as substrate

Homo sapiens

2.7.1.21

0.00079

-

dCTP

wild-type enzyme, pH 7.6, competitive with thymidine as substrate

Homo sapiens

2.7.1.21

0.00087

-

dCTP

wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate

Homo sapiens

2.7.1.21

0.00089

-

dCTP

H121N mutant, pH 7.6, competitive with deoxycytidine as substrate

Homo sapiens

2.7.1.21

0.00089

-

dTTP

H121N mutant, pH 7.6, competitive with thymidine as substrate

Homo sapiens

2.7.1.21

0.002

-

dTTP

wild-type enzyme, pH 7.6, competitive with thymidine as substrate

Homo sapiens

2.7.1.21

0.0025

-

dTTP

wild-type and H121N mutant, pH 7.6, non-competitive with deoxycytidine as substrate

Homo sapiens

2.7.1.21

0.0036

-

thymidine

H121N mutant, pH 7.6, competitive with deoxycytidine as substrate

Homo sapiens

2.7.1.21

0.004

-

deoxycytidine

H121N mutant, pH 7.6, non-competitive with thymidine as substrate

Homo sapiens

2.7.1.21

0.0049

-

thymidine

wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate

Homo sapiens

2.7.1.21

0.04

-

deoxycytidine

wild-type enzyme, pH 7.6, non-competitive with thymidine as substrate

Homo sapiens

EC Number

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

2.7.1.21

0.0057

-

thymidine

H121N mutant, pH 7.6

Homo sapiens

2.7.1.21

0.011

-

deoxycytidine

wild-type and H121N mutant, pH 7.6

Homo sapiens

2.7.1.21

0.013

-

thymidine

wild-type, pH 7.6

Homo sapiens

2.7.1.21

0.018

-

thymidine

I212 mutant, pH 7.6

Homo sapiens

2.7.1.21

0.025

-

ATP

wild-type, thymidine as substrate, pH 7.6

Homo sapiens

2.7.1.21

0.037

-

ATP

wild-type, deoxycytidine as substrate, pH 7.6

Homo sapiens

2.7.1.21

0.059

-

ATP

I212 mutant, deoxycytidine as substrate, pH 7.6

Homo sapiens

2.7.1.21

0.08

-

ATP

H121N mutant, thymidine as substrate, pH 7.6

Homo sapiens

2.7.1.21

0.095

-

ATP

I212 mutant, thymidine as substrate, pH 7.6

Homo sapiens

2.7.1.21

0.162

-

ATP

H121N mutant, deoxycytidine as substrate, pH 7.6

Homo sapiens

2.7.1.21

0.568

-

deoxycytidine

I212 mutant, pH 7.6

Homo sapiens

EC Number

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2.7.1.21

ATP + 2'-deoxycytidine

-

641150

Homo sapiens

ADP + 2'-deoxycytidine 5'-phosphate

-

-

-

-