BRENDA - Enzyme Database show

Kinetic properties of mutant human thymidine kinase 2 suggest a mechanism for mitochondrial DNA depletion myopathy

Wang, L.; Saada, A.; Eriksson, S.; J. Biol. Chem. 278, 6963-6968 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.21
TK2
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.21
H121N
similar subunit structure to wild-type enzyme, decreased enzyme activity
Homo sapiens
2.7.1.21
I212N
similar subunit structure to wild-type enzyme, less than 1% activity compared to wild-type
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.21
dCTP
-
Homo sapiens
2.7.1.21
deoxcytidine
-
Homo sapiens
2.7.1.21
dTTP
-
Homo sapiens
2.7.1.21
thymidine
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
0.0057
-
thymidine
H121N mutant, pH 7.6
Homo sapiens
2.7.1.21
0.011
-
deoxycytidine
wild-type and H121N mutant, pH 7.6
Homo sapiens
2.7.1.21
0.013
-
thymidine
wild-type, pH 7.6
Homo sapiens
2.7.1.21
0.018
-
thymidine
I212 mutant, pH 7.6
Homo sapiens
2.7.1.21
0.025
-
ATP
wild-type, thymidine as substrate, pH 7.6
Homo sapiens
2.7.1.21
0.037
-
ATP
wild-type, deoxycytidine as substrate, pH 7.6
Homo sapiens
2.7.1.21
0.059
-
ATP
I212 mutant, deoxycytidine as substrate, pH 7.6
Homo sapiens
2.7.1.21
0.08
-
ATP
H121N mutant, thymidine as substrate, pH 7.6
Homo sapiens
2.7.1.21
0.095
-
ATP
I212 mutant, thymidine as substrate, pH 7.6
Homo sapiens
2.7.1.21
0.162
-
ATP
H121N mutant, deoxycytidine as substrate, pH 7.6
Homo sapiens
2.7.1.21
0.568
-
deoxycytidine
I212 mutant, pH 7.6
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.21
mitochondrion
-
Homo sapiens
5739
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.21
Homo sapiens
O00142
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + 2'-deoxycytidine
-
641150
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
-
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.21
0.00036
-
dCTP
H121N mutant, pH 7.6, competitive with thymidine as substrate
Homo sapiens
2.7.1.21
0.00079
-
dCTP
wild-type enzyme, pH 7.6, competitive with thymidine as substrate
Homo sapiens
2.7.1.21
0.00087
-
dCTP
wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate
Homo sapiens
2.7.1.21
0.00089
-
dCTP
H121N mutant, pH 7.6, competitive with deoxycytidine as substrate
Homo sapiens
2.7.1.21
0.00089
-
dTTP
H121N mutant, pH 7.6, competitive with thymidine as substrate
Homo sapiens
2.7.1.21
0.002
-
dTTP
wild-type enzyme, pH 7.6, competitive with thymidine as substrate
Homo sapiens
2.7.1.21
0.0025
-
dTTP
wild-type and H121N mutant, pH 7.6, non-competitive with deoxycytidine as substrate
Homo sapiens
2.7.1.21
0.0036
-
thymidine
H121N mutant, pH 7.6, competitive with deoxycytidine as substrate
Homo sapiens
2.7.1.21
0.004
-
deoxycytidine
H121N mutant, pH 7.6, non-competitive with thymidine as substrate
Homo sapiens
2.7.1.21
0.0049
-
thymidine
wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate
Homo sapiens
2.7.1.21
0.04
-
deoxycytidine
wild-type enzyme, pH 7.6, non-competitive with thymidine as substrate
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.21
TK2
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.21
H121N
similar subunit structure to wild-type enzyme, decreased enzyme activity
Homo sapiens
2.7.1.21
I212N
similar subunit structure to wild-type enzyme, less than 1% activity compared to wild-type
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.21
dCTP
-
Homo sapiens
2.7.1.21
deoxcytidine
-
Homo sapiens
2.7.1.21
dTTP
-
Homo sapiens
2.7.1.21
thymidine
-
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.21
0.00036
-
dCTP
H121N mutant, pH 7.6, competitive with thymidine as substrate
Homo sapiens
2.7.1.21
0.00079
-
dCTP
wild-type enzyme, pH 7.6, competitive with thymidine as substrate
Homo sapiens
2.7.1.21
0.00087
-
dCTP
wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate
Homo sapiens
2.7.1.21
0.00089
-
dCTP
H121N mutant, pH 7.6, competitive with deoxycytidine as substrate
Homo sapiens
2.7.1.21
0.00089
-
dTTP
H121N mutant, pH 7.6, competitive with thymidine as substrate
Homo sapiens
2.7.1.21
0.002
-
dTTP
wild-type enzyme, pH 7.6, competitive with thymidine as substrate
Homo sapiens
2.7.1.21
0.0025
-
dTTP
wild-type and H121N mutant, pH 7.6, non-competitive with deoxycytidine as substrate
Homo sapiens
2.7.1.21
0.0036
-
thymidine
H121N mutant, pH 7.6, competitive with deoxycytidine as substrate
Homo sapiens
2.7.1.21
0.004
-
deoxycytidine
H121N mutant, pH 7.6, non-competitive with thymidine as substrate
Homo sapiens
2.7.1.21
0.0049
-
thymidine
wild-type enzyme, pH 7.6, competitive with deoxycytidine as substrate
Homo sapiens
2.7.1.21
0.04
-
deoxycytidine
wild-type enzyme, pH 7.6, non-competitive with thymidine as substrate
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
0.0057
-
thymidine
H121N mutant, pH 7.6
Homo sapiens
2.7.1.21
0.011
-
deoxycytidine
wild-type and H121N mutant, pH 7.6
Homo sapiens
2.7.1.21
0.013
-
thymidine
wild-type, pH 7.6
Homo sapiens
2.7.1.21
0.018
-
thymidine
I212 mutant, pH 7.6
Homo sapiens
2.7.1.21
0.025
-
ATP
wild-type, thymidine as substrate, pH 7.6
Homo sapiens
2.7.1.21
0.037
-
ATP
wild-type, deoxycytidine as substrate, pH 7.6
Homo sapiens
2.7.1.21
0.059
-
ATP
I212 mutant, deoxycytidine as substrate, pH 7.6
Homo sapiens
2.7.1.21
0.08
-
ATP
H121N mutant, thymidine as substrate, pH 7.6
Homo sapiens
2.7.1.21
0.095
-
ATP
I212 mutant, thymidine as substrate, pH 7.6
Homo sapiens
2.7.1.21
0.162
-
ATP
H121N mutant, deoxycytidine as substrate, pH 7.6
Homo sapiens
2.7.1.21
0.568
-
deoxycytidine
I212 mutant, pH 7.6
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.21
mitochondrion
-
Homo sapiens
5739
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + 2'-deoxycytidine
-
641150
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
-