Literature summary extracted from

Kit, S.; Leung, W.C.; Trkula, D.
Properties of mitochondrial thymidine kinases of parental and enzyme-deficient HeLa cells (1973), Arch. Biochem. Biophys., 158, 503-513.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.7.1.21	dCTP	30-45% inhibition of mitochondrial enzyme at 0.6 or 1 mM, no inhibition of cytosolic enzyme	Homo sapiens
2.7.1.21	dTTP	-	Homo sapiens
2.7.1.21	additional information	5'-derivatives of thymidine	Homo sapiens

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.7.1.21	cytosol	-	Homo sapiens	5829	-
2.7.1.21	mitochondrion	-	Homo sapiens	5739	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.7.1.21	Mg2+	-	Homo sapiens

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.7.1.21	70000	74000	mitochondria, glycerol gradient centrifugation	Homo sapiens
2.7.1.21	84000	98000	cytoplasm, glycerol density gradient centrifugation	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.1.21	Homo sapiens	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.7.1.21	HeLa cell	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.7.1.21	additional information	-	-	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.1.21	ATP + thymidine	ATP can be substituted by dGTP	Homo sapiens	ADP + dTMP	-	?

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Release 2023.1 (January 2023)