Literature summary extracted from

Cheng, Y.C.; Ostrander, M.
Deoxythymidine kinase induced in HeLa TK- cells by Herpes simplex virus type I and type II. II. Purification and characterization (1976), J. Biol. Chem., 251, 2605-2610.

General Stability

EC Number	General Stability	Organism
2.7.1.21	ATP or thymidine stabilizes, type I more stable in the presence of MgATP2- than type II	Herpes simplex virus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.7.1.21	ammoniumsulfate	50% inhibition at 200 mM	Herpes simplex virus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.7.1.21	cytoplasm	-	Herpes simplex virus	5737	-
2.7.1.21	nucleus	-	Herpes simplex virus	5634	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.1.21	Herpes simplex virus	-	type 2	-
2.7.1.21	Herpes simplex virus	-	type 1	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.1.21	HeLa TK- cells infected with virus	Herpes simplex virus

Storage Stability

EC Number	Storage Stability	Organism
2.7.1.21	-70°C, 0.2 mM deoxythymidine, 3 mM dithiothreitol, 10% glycerol	Herpes simplex virus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.1.21	ATP + thymidine	-	Herpes simplex virus	ADP + dTMP	-	?

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
2.7.1.21	27	43	stable	Herpes simplex virus
2.7.1.21	45	-	half-life 28 min, Herpes simplex virus type 2: half-life 120 min	Herpes simplex virus

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Release 2023.1 (January 2023)