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Literature summary extracted from

  • Ellims, P.H.; van der Weyden, M.B.
    Human liver thymidine kinase. Purification and some properties of the enzyme (1980), J. Biol. Chem., 255, 11290-11295.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.21 not stabilized by 10% glycerol, 5 mM 2-mercaptoethanol, 5 mM EDTA, 1 mg/ml bovine serum albumin Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.21 cytosol
-
 Homo sapiens 5829
-
2.7.1.21 mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 48000
-
 2 * 48000, SDS-PAGE Homo sapiens
2.7.1.21 49000
-
 gel filtration Homo sapiens
2.7.1.21 340000
-
 oligomeric form, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 rLyTK1Val-106 and rLyTK1Met-106 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.21 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 additional information
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.1.21 -20°C, or 4°C, 24 h, more than 50% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + thymidine
-
 Homo sapiens ADP + dTMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 dimer 2 * 48000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.21 40
-
 enzyme TK-A: half-life 8 h, enzyme TK-C: half-life 30 min Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.21 7.4
-
 bimodal pH profile with a peak at pH 7.4 and a minor peak at pH 9.0 Homo sapiens
2.7.1.21 9
-
 bimodal pH profile with a peak at pH 7.4 and a minor peak at pH 9.0 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.21 Homo sapiens linear pH gradient
-
 5.1