Literature summary extracted from

Sandlie, I.; Kleppe, K.
Mechanism of inhibition of thymidine kinase from Escherichia coli by caffeine (1980), FEBS Lett., 110, 223-226.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.7.1.21	dCDP	activation	Escherichia coli
2.7.1.21	dCTP	activation	Escherichia coli

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.7.1.21	adenine	-	Escherichia coli
2.7.1.21	Caffeine	competitive to ATP, 75% inhibition at 20 mM	Escherichia coli
2.7.1.21	theophylline	-	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.1.21	Escherichia coli	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.1.21	ATP + thymidine	ATP can be substituted by dATP	Escherichia coli	ADP + dTMP	-	?

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.7.1.21	7.5	-	Caffeine	pH 7.8, 37°C	Escherichia coli

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Release 2023.1 (January 2023)