BRENDA - Enzyme Database show

Kinetic mechanism and inhibition of human liver thymidine kinase

Ellims, P.H.; van der Weyden, M.B.; Biochim. Biophys. Acta 660, 238-242 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.21
ADP
-
Homo sapiens
2.7.1.21
additional information
overview: purine nucleotides, pyrimidine nucleotides
Homo sapiens
2.7.1.21
TMP
-
Homo sapiens
2.7.1.21
TTP
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
0.005
-
thymidine
pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.006
-
deoxycytidine
pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.09
-
ATP
pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.15
-
CTP
pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.26
-
UTP
pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.3
-
GTP
pH 7.4, 37°C
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.21
Mg2+
-
Homo sapiens
2.7.1.21
Mn2+
can partially substitute for Mg2+
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.21
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.21
liver
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + 2'-deoxycytidine
-
641131
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + thymidine
ATP can be substituted by UTP
641131
Homo sapiens
ADP + dTMP
-
-
-
?
2.7.1.21
CTP + thymidine
-
641131
Homo sapiens
CDP + thymidine 5'-phosphate
-
-
-
-
2.7.1.21
GTP + thymidine
-
641131
Homo sapiens
GDP + thymidine 5'-phosphate
-
-
-
-
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.21
0.0085
-
TTP
competitive to ATP, pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.0135
-
TTP
competitive to thymidine, pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.63
-
TMP
pH 7.4, 37°C
Homo sapiens
2.7.1.21
6.5
-
ADP
pH 7.4, 37°C
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.21
ADP
-
Homo sapiens
2.7.1.21
additional information
overview: purine nucleotides, pyrimidine nucleotides
Homo sapiens
2.7.1.21
TMP
-
Homo sapiens
2.7.1.21
TTP
-
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.21
0.0085
-
TTP
competitive to ATP, pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.0135
-
TTP
competitive to thymidine, pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.63
-
TMP
pH 7.4, 37°C
Homo sapiens
2.7.1.21
6.5
-
ADP
pH 7.4, 37°C
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
0.005
-
thymidine
pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.006
-
deoxycytidine
pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.09
-
ATP
pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.15
-
CTP
pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.26
-
UTP
pH 7.4, 37°C
Homo sapiens
2.7.1.21
0.3
-
GTP
pH 7.4, 37°C
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.21
Mg2+
-
Homo sapiens
2.7.1.21
Mn2+
can partially substitute for Mg2+
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.21
liver
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + 2'-deoxycytidine
-
641131
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + thymidine
ATP can be substituted by UTP
641131
Homo sapiens
ADP + dTMP
-
-
-
?
2.7.1.21
CTP + thymidine
-
641131
Homo sapiens
CDP + thymidine 5'-phosphate
-
-
-
-
2.7.1.21
GTP + thymidine
-
641131
Homo sapiens
GDP + thymidine 5'-phosphate
-
-
-
-