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Literature summary extracted from

  • Chraibi, R.; Wright, M.
    Characterization of the thymidine kinase of Physarum polycephalum (1983), J. Biochem., 93, 323-330.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 5-bromodeoxyuridine competitive to thymidine Physarum polycephalum
2.7.1.21 5-fluorodeoxyuridine competitive to thymidine Physarum polycephalum
2.7.1.21 5-iododeoxyuridine competitive to thymidine Physarum polycephalum
2.7.1.21 ADP competitive to ATP Physarum polycephalum
2.7.1.21 Caffeine competitive to ATP Physarum polycephalum
2.7.1.21 deoxyuridine
-
Physarum polycephalum
2.7.1.21 dGDP competitive to ATP Physarum polycephalum
2.7.1.21 dTDP competitive to ATP Physarum polycephalum
2.7.1.21 dTMP competitive to ATP Physarum polycephalum
2.7.1.21 dTTP non-competitive with thymidine, competitive to ATP Physarum polycephalum
2.7.1.21 dUTP competitive to ATP Physarum polycephalum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 additional information
-
additional information
-
Physarum polycephalum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.21 Mg2+ 7.5-10 mM, required Physarum polycephalum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Physarum polycephalum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21
-
Physarum polycephalum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.21 plasmodium
-
Physarum polycephalum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 100
-
-
Physarum polycephalum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + thymidine ATP can be substituted by dATP Physarum polycephalum ADP + dTMP
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.21 7.5 9
-
Physarum polycephalum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.21 7 9.5 less than 70% of maximal activity above and below Physarum polycephalum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.21 0.001 0.0045 5-iododeoxyuridine pH 7.5, 27°C Physarum polycephalum
2.7.1.21 0.0015 0.0055 5-bromodeoxyuridine pH 7.5, 27°C Physarum polycephalum
2.7.1.21 0.014 0.036 dTTP pH 7.5, 27°C Physarum polycephalum
2.7.1.21 0.06 0.096 5-fluorodeoxyuridine pH 7.5, 27°C Physarum polycephalum
2.7.1.21 0.27 1.54 deoxyuridine pH 7.5, 27°C Physarum polycephalum
2.7.1.21 0.5 0.65 dTDP pH 7.5, 27°C Physarum polycephalum
2.7.1.21 3.6 4.7 dTMP pH 7.5, 27°C Physarum polycephalum