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Literature summary extracted from

  • Cheng, Y.C.
    Thymidine kinase from blast cells of myelocytic leukemia (1978), Methods Enzymol., 51, 365-371.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.21 bovine serum albumin, 0.5 mg/ml stabilizes Homo sapiens
2.7.1.21 dithiothreitol essential for stability Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 dCTP mitochondrial enzyme Homo sapiens
2.7.1.21 dTTP
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.0026
-
thymidine cytoplasmic enzyme, pH 7.5, 37°C Homo sapiens
2.7.1.21 0.003
-
deoxycytidine mitochondrial enzyme, pH 7.5, 37°C Homo sapiens
2.7.1.21 0.005 0.0052 thymidine mitochondrial enzyme, pH 7.5, 37°C Homo sapiens
2.7.1.21 0.1
-
ATP mitochondrial enzyme, pH 7.5, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.21 cytoplasm
-
Homo sapiens 5737
-
2.7.1.21 mitochondrion
-
Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.21 Ca2+ can partially substitute for Mg2+ Homo sapiens
2.7.1.21 Cu2+ can partially substitute for Mg2+ Homo sapiens
2.7.1.21 Fe2+ can partially substitute for Mg2+ Homo sapiens
2.7.1.21 Mg2+
-
Homo sapiens
2.7.1.21 Mn2+ can partially substitute for Mg2+ Homo sapiens
2.7.1.21 Zn2+ can partially substitute for Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 70000 74000 mitochondria, glycerol gradient centrifugation Homo sapiens
2.7.1.21 84000 98000 cytoplasm, glycerol density gradient centrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 rLyTK1Val-106 and rLyTK1Met-106 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.21 blast cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + 2'-deoxycytidine
-
Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
?
2.7.1.21 ATP + 5-bromo-2'-deoxyuridine
-
Homo sapiens ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
?
2.7.1.21 ATP + 5-iodo-2'-deoxyuridine
-
Homo sapiens ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
?
2.7.1.21 ATP + thymidine ATP can be substituted by dATP Homo sapiens ADP + dTMP
-
?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.21 0.002
-
dCTP pH 7.5, 37°C Homo sapiens
2.7.1.21 0.01
-
dTTP pH 7.5, 37°C Homo sapiens