BRENDA - Enzyme Database show

Thymidine kinase from blast cells of myelocytic leukemia

Cheng, Y.C.; Methods Enzymol. 51, 365-371 (1978)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.1.21
bovine serum albumin, 0.5 mg/ml stabilizes
Homo sapiens
2.7.1.21
dithiothreitol essential for stability
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.21
dCTP
mitochondrial enzyme
Homo sapiens
2.7.1.21
dTTP
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
0.0026
-
thymidine
cytoplasmic enzyme, pH 7.5, 37°C
Homo sapiens
2.7.1.21
0.003
-
deoxycytidine
mitochondrial enzyme, pH 7.5, 37°C
Homo sapiens
2.7.1.21
0.005
0.0052
thymidine
mitochondrial enzyme, pH 7.5, 37°C
Homo sapiens
2.7.1.21
0.1
-
ATP
mitochondrial enzyme, pH 7.5, 37°C
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.21
cytoplasm
-
Homo sapiens
5737
-
2.7.1.21
mitochondrion
-
Homo sapiens
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.21
Ca2+
can partially substitute for Mg2+
Homo sapiens
2.7.1.21
Cu2+
can partially substitute for Mg2+
Homo sapiens
2.7.1.21
Fe2+
can partially substitute for Mg2+
Homo sapiens
2.7.1.21
Mg2+
-
Homo sapiens
2.7.1.21
Mn2+
can partially substitute for Mg2+
Homo sapiens
2.7.1.21
Zn2+
can partially substitute for Mg2+
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.21
70000
74000
mitochondria, glycerol gradient centrifugation
Homo sapiens
2.7.1.21
84000
98000
cytoplasm, glycerol density gradient centrifugation
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.21
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.21
rLyTK1Val-106 and rLyTK1Met-106
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.21
blast cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.21
additional information
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + 2'-deoxycytidine
-
641125
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
-
2.7.1.21
ATP + 5-bromo-2'-deoxyuridine
-
641125
Homo sapiens
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + 5-iodo-2'-deoxyuridine
-
641125
Homo sapiens
ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + thymidine
ATP can be substituted by dATP
641125
Homo sapiens
ADP + dTMP
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.21
0.002
-
dCTP
pH 7.5, 37°C
Homo sapiens
2.7.1.21
0.01
-
dTTP
pH 7.5, 37°C
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.21
bovine serum albumin, 0.5 mg/ml stabilizes
Homo sapiens
2.7.1.21
dithiothreitol essential for stability
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.21
dCTP
mitochondrial enzyme
Homo sapiens
2.7.1.21
dTTP
-
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.21
0.002
-
dCTP
pH 7.5, 37°C
Homo sapiens
2.7.1.21
0.01
-
dTTP
pH 7.5, 37°C
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.21
0.0026
-
thymidine
cytoplasmic enzyme, pH 7.5, 37°C
Homo sapiens
2.7.1.21
0.003
-
deoxycytidine
mitochondrial enzyme, pH 7.5, 37°C
Homo sapiens
2.7.1.21
0.005
0.0052
thymidine
mitochondrial enzyme, pH 7.5, 37°C
Homo sapiens
2.7.1.21
0.1
-
ATP
mitochondrial enzyme, pH 7.5, 37°C
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.21
cytoplasm
-
Homo sapiens
5737
-
2.7.1.21
mitochondrion
-
Homo sapiens
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.21
Ca2+
can partially substitute for Mg2+
Homo sapiens
2.7.1.21
Cu2+
can partially substitute for Mg2+
Homo sapiens
2.7.1.21
Fe2+
can partially substitute for Mg2+
Homo sapiens
2.7.1.21
Mg2+
-
Homo sapiens
2.7.1.21
Mn2+
can partially substitute for Mg2+
Homo sapiens
2.7.1.21
Zn2+
can partially substitute for Mg2+
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.21
70000
74000
mitochondria, glycerol gradient centrifugation
Homo sapiens
2.7.1.21
84000
98000
cytoplasm, glycerol density gradient centrifugation
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.21
rLyTK1Val-106 and rLyTK1Met-106
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.21
blast cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.21
additional information
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.21
ATP + 2'-deoxycytidine
-
641125
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
-
2.7.1.21
ATP + 5-bromo-2'-deoxyuridine
-
641125
Homo sapiens
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + 5-iodo-2'-deoxyuridine
-
641125
Homo sapiens
ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
-
-
?
2.7.1.21
ATP + thymidine
ATP can be substituted by dATP
641125
Homo sapiens
ADP + dTMP
-
-
-
?