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Literature summary extracted from

  • Chen, M.S.; Prusoff, W.H.
    Thymidine kinase from Escherichia coli (1978), Methods Enzymol., 51, 354-360.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.21 dCTP activation Escherichia coli
2.7.1.21 GTP activation Escherichia coli
2.7.1.21 Hydroxymethyl-dCDP activation Escherichia coli
2.7.1.21 Hydroxymethyl-dCTP activation Escherichia coli
2.7.1.21 additional information Km values of allosteric regulators Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 3-N-methyl-5-iodo-2'-deoxyuridine
-
 Escherichia coli
2.7.1.21 dTTP
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.0012
-
 5-iodo-2'-deoxyuridine
-
 Escherichia coli
2.7.1.21 0.0017
-
 deoxythymidine
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.21 Mg2+
-
 Escherichia coli
2.7.1.21 Mn2+ can partially substitute for Mg2+ Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 720
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.1.21 -70°C, 10 mM 2-mercaptoethanol, 0.5 mg/ml bovine serum albumin, several months Escherichia coli
2.7.1.21 0°C, 2-3 months, change of kinetic properties, larger amount of ATP is required to get the same rate of reaction Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + 5-bromo-2'-deoxyuridine ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP Escherichia coli ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-chloro-2'-deoxyuridine ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP Escherichia coli ADP + 5-chloro-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-fluoro-2'-deoxyuridine ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP Escherichia coli ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-iodo-2'-deoxyuridine ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP Escherichia coli ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 5-mercapto-2'-deoxyuridine ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP Escherichia coli ADP + 5-mercapto-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + deoxythymidine ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP Escherichia coli ADP + dTMP
-
 ?
2.7.1.21 ATP + deoxyuridine ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP Escherichia coli ADP + dUMP
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.21 7.5
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.21 dATP activation Escherichia coli
2.7.1.21 dGTP activation Escherichia coli
2.7.1.21 GDP activation Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.21 1.7
-
 3-N-methyl-5-iodo-2'-deoxyuridine pH 7.8, 37°C Escherichia coli