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Literature summary extracted from

  • Sherley, J.L.; Kelly, T.J.
    Human cytosolic thymidine kinase. Purification and physical characterization of the enzyme from HeLa cells (1988), J. Biol. Chem., 263, 375-382.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.21 digitonin stabilizes Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.21 cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 24000
-
 2 * 24000, cytosol, SDS-PAGE Homo sapiens
2.7.1.21 84000 98000 calculation from Stokes' radius and sedimentation coefficient Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.21 ATP + thymidine Homo sapiens involved in DNA replication ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 rLyTK1Val-106 and rLyTK1Met-106 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.21 HeLa cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 325
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.1.21 -80°C, 2 weeks, no loss of activity in presence of digitonin Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + thymidine
-
 Homo sapiens ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine involved in DNA replication Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 dimer 2 * 24000, cytosol, SDS-PAGE Homo sapiens