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Literature summary extracted from

  • Tsukamoto, I.; Taniguchi, Y.; Miyoshi, M.; Kojo, S.
    Purification and characterization of thymidine kinase from regenerating rat liver (1991), Biochim. Biophys. Acta, 1079, 348-352.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.21 dithiothreitol, 5 mM stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 ADP competitive to ATP Rattus norvegicus
2.7.1.21 dTMP competitive to thymidine Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.0022
-
 thymidine pH 8, 37°C Rattus norvegicus
2.7.1.21 0.2
-
 ATP pH 8, 37°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 26000
-
 2 * 26000, SDS-PAGE Rattus norvegicus
2.7.1.21 54000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21 homogeneity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.21 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.21 14
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + thymidine ATP can be substituted by GTP Rattus norvegicus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by dGTP Rattus norvegicus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by UTP Rattus norvegicus ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine ATP can be substituted by dATP Rattus norvegicus ADP + dTMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 dimer 2 * 26000, SDS-PAGE Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.21 0.4
-
 ADP with ATP as variable substrate, pH 8, 37°C Rattus norvegicus
2.7.1.21 0.65
-
 dTMP with ATP or thymidine as variable substrate, pH 8, 37°C Rattus norvegicus