Literature summary extracted from

Kizer, D.E.; Hollman, L.
Purification and properties of thymidine kinase from regenerating rat liver (1974), Biochim. Biophys. Acta, 350, 193-200.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.7.1.21	dTTP	-	Rattus norvegicus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.7.1.21	0.0032	-	thymidine	-	Rattus norvegicus
2.7.1.21	0.3	-	ATP	-	Rattus norvegicus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.7.1.21	69000	-	sucrose density gradient centrifugation	Rattus norvegicus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.1.21	Rattus norvegicus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.1.21	2600fold	Rattus norvegicus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.7.1.21	liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.7.1.21	additional information	-	-	Rattus norvegicus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.1.21	ATP + thymidine	-	Rattus norvegicus	ADP + dTMP	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.7.1.21	7.5	9	-	Rattus norvegicus

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
2.7.1.21	5	10.2	less than 50% of maximal activity above and below	Rattus norvegicus

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Release 2023.1 (January 2023)