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Literature summary extracted from

  • Bhaumik, D.; Datta, A.K.
    Immunochemical and catalytic characteristics of adenosine kinase from Leishmania donovani (1989), J. Biol. Chem., 264, 4356-4361.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 adenosine no inhibition; strong, with formycin A as substrate Leishmania donovani
2.7.1.20 Formycin A at very high concentrations, kinetics Leishmania donovani
2.7.1.20 NEM moderate inhibition; strong inhibition with formycin A as substrate, adenosine protects Leishmania donovani
2.7.1.20 Polyclonal antibodies to adenosine kinase from Leishmania donovani not from other eukaryotic sources Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 0.016
-
 adenosine
-
 Leishmania donovani
2.7.1.20 0.016
-
 adenosine pH 7.5, 30ºC Leishmania donovani
2.7.1.20 0.02 0.024 ATP formycin A Leishmania donovani
2.7.1.20 0.02 0.024 ATP ATP in form of MgATP2- Leishmania donovani
2.7.1.20 0.024
-
 Formycin A pH 7.5, 30ºC Leishmania donovani

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 KCl activation, formycin A as substrate. Adenosine phosphorylation is neither stimulated nor inhibited Leishmania donovani
2.7.1.20 Mg2+ requirement Leishmania donovani
2.7.1.20 Mg2+ reactive species: MgATP2- Leishmania donovani

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Leishmania donovani
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + adenosine
-
 Leishmania donovani ADP + AMP
-
 ?
2.7.1.20 ATP + formycin A
-
 Leishmania donovani ADP + formycin A 5'-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.20 30
-
 assay at Leishmania donovani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.20 24
-
 adenosine formycin A Leishmania donovani