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Literature summary extracted from

  • Chang, C.H.; Brockman, R.W.; Bennett, L.L.
    Adenosine kinase from L1210 cells. Purification and some properties of the enzyme (1980), J. Biol. Chem., 255, 2366-2371.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.20 bovine serum albumin
-
 Mus musculus
2.7.1.20 additional information no activation by polyamines Mus musculus

General Stability

EC Number General Stability Organism
2.7.1.20 bovine serum albumin stabilizes Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 Mg2+ above 0.7 mM Mus musculus
2.7.1.20 additional information no inhibition by polyamines Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 0.0017
-
 adenosine pH 6.8, 37ºC, 12 mM ATP and 3 mM MgCl2 Mus musculus
2.7.1.20 0.002
-
 adenosine pH 6.8, 37ºC, 1 mM ATP and 0.7 mM MgCl2 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.20 cytosol
-
 Mus musculus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Ca2+ activation Mus musculus
2.7.1.20 Co2+ can partially substitute for Mg2+ Mus musculus
2.7.1.20 Co2+ activation Mus musculus
2.7.1.20 Fe2+ activation Mus musculus
2.7.1.20 Fe2+ sulfate form is more effective than the chloride form Mus musculus
2.7.1.20 Mg2+ requirement Mus musculus
2.7.1.20 Mg2+ the chloride form is more effective than the sulfate form Mus musculus
2.7.1.20 Mn2+ can partially substitute for Mg2+ Mus musculus
2.7.1.20 Mn2+ activation Mus musculus
2.7.1.20 Ni2+ can partially substitute for Mg2+ Mus musculus
2.7.1.20 Ni2+ activation Mus musculus
2.7.1.20 Zn2+ no activation Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.20 56000
-
 PAGE, sucrose density gradient centrifugation, gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 streptomycin sulfate and ammonium sulfate fractionation, chromatography on DEAE-cellulose and affinity chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.20 Ehrlich ascites carcinoma cell
-
 Mus musculus
-
2.7.1.20 leukemia cell L1210, implanted in mice Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.20 0.305
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.7.1.20 -20°C, in 45% sucrose, at least 1 month Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + adenosine strictly specific for adenosine Mus musculus ADP + AMP
-
 ?
2.7.1.20 CTP + adenosine CTP is a poor substrate Mus musculus CDP + AMP
-
 ?
2.7.1.20 GTP + adenosine best substrate Mus musculus GDP + AMP
-
 ?
2.7.1.20 ITP + adenosine about half as effective as GTP Mus musculus IDP + AMP
-
 ?
2.7.1.20 ITP + adenosine about half as effective as ATP Mus musculus IDP + AMP
-
 ?
2.7.1.20 UTP + adenosine UTP is a poor substrate Mus musculus UDP + AMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.20 37
-
 assay at Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.20 25 37 60 min stable at pH 6.8 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.20 additional information
-
 the pH-optimum is a function of the ATP/Mg2+ ratio Mus musculus
2.7.1.20 6.8
-
-
 Mus musculus