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Literature summary extracted from

  • Yamada, Y.; Goto, H.; Ogasawara, N.
    Purification and properties of adenosine kinase from rat brain (1980), Biochim. Biophys. Acta, 616, 199-207.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.20 0.15 M KCl stabilizes, rapid inactivation at low salt concentrations, DTT reverses Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 adenosine above 0.0005 mM Rattus norvegicus
2.7.1.20 Cu2+
-
 Rattus norvegicus
2.7.1.20 EDTA
-
 Rattus norvegicus
2.7.1.20 Mg2+ above 0.4 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 0.00015 0.0005 adenosine pH 7.5, 30ºC Rattus norvegicus
2.7.1.20 0.006
-
 ATP pH 7.5, 30ºC, with deoxyadenosine as substrate Rattus norvegicus
2.7.1.20 0.02
-
 ATP pH 7.5, 30ºC Rattus norvegicus
2.7.1.20 0.1
-
 MgCl2 pH 7.5, 30ºC Rattus norvegicus
2.7.1.20 0.25
-
 ATP pH 7.5, 30ºC, with deoxyadenosine as substrate Rattus norvegicus
2.7.1.20 0.36 0.67 2'-deoxy-adenosine pH 7.5, 30ºC Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Ba2+ no activation Rattus norvegicus
2.7.1.20 Ca2+ can partially substitute for Mg2+ Rattus norvegicus
2.7.1.20 Ca2+ activation Rattus norvegicus
2.7.1.20 Co2+ activation Rattus norvegicus
2.7.1.20 Co2+ as efective as ATP Rattus norvegicus
2.7.1.20 Fe2+ no activation Rattus norvegicus
2.7.1.20 Mg2+ activation Rattus norvegicus
2.7.1.20 Mn2+ can partially substitute for Mg2+ Rattus norvegicus
2.7.1.20 Mn2+ activation Rattus norvegicus
2.7.1.20 additional information no activation by Zn2+ and Ba2+ Rattus norvegicus
2.7.1.20 additional information no activation by Cr2+ Rattus norvegicus
2.7.1.20 additional information no activation by Fe2+ Rattus norvegicus
2.7.1.20 Ni2+ can partially substitute for Mg2+ Rattus norvegicus
2.7.1.20 Ni2+ activation Rattus norvegicus
2.7.1.20 Zn2+ no activation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.20 38000
-
 gel filtration Rattus norvegicus
2.7.1.20 41000
-
 7.5% acrylamide gel electrophoresis in the presence of SDS Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 ammonium sulfate fractionation, affinity chromatography and gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.20 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.20 7.8
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + 2'-deoxy-adenosine
-
 Rattus norvegicus ADP + 2'-deoxy-AMP
-
 ?
2.7.1.20 ATP + adenosine
-
 Rattus norvegicus ADP + AMP
-
 ?
2.7.1.20 GTP + adenosine more effective than ATP Rattus norvegicus GDP + AMP
-
 ?
2.7.1.20 UTP + adenosine
-
 Rattus norvegicus UDP + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.20 monomer 1 * 41000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.20 30
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.20 additional information
-
 biphasic pH-profile with adenosine, monophasic with deoxyadenosine Rattus norvegicus
2.7.1.20 5.5
-
 sharp, adenosine as substrate Rattus norvegicus
2.7.1.20 6
-
 monophasic, adenosine 0.025 mM Rattus norvegicus
2.7.1.20 7.5 8.5 broad, adenosine or deoxyadenosine as substrates Rattus norvegicus