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Literature summary extracted from

  • Drabikowska, A.K.; Halec, L.; Shugar, D.
    Purification and properties of adenosine kinase from rat liver: separation from deoxyadenosine kinase activity (1985), Z. Naturforsch. C, 40, 34-41.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.20 at 1 mg protein/ml stable upon repeated freeze-thawing Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 adenosine 0.001 mM Rattus norvegicus
2.7.1.20 tubercidin
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 additional information
-
 additional information pH and Mg/ATP ratio-dependent Km Rattus norvegicus
2.7.1.20 0.00015 0.0005 adenosine
-
 Rattus norvegicus
2.7.1.20 0.36 0.67 2'-deoxy-adenosine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.20 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Mg2+ requirement Rattus norvegicus
2.7.1.20 Mg2+ reactive species: MgATP2- Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.20 52000
-
 1 * 52000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Rattus norvegicus
-
-
-
2.7.1.76 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.76 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.20 18
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.20 -25°C, 1 mg protein/ml, several months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + 2'-deoxy-adenosine
-
 Rattus norvegicus ADP + 2'-deoxy-AMP
-
 ?
2.7.1.20 ATP + adenosine
-
 Rattus norvegicus ADP + AMP
-
 ?
2.7.1.76 ATP + deoxyadenosine
-
 Rattus norvegicus ADP + dAMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.20 monomer 1 * 52000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.76 More enzyme may be identical with EC 2.7.1.20, i.e. adenosine kinase Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.20 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.20 additional information
-
 the pH-optimum is a function of the adenosine concentration Rattus norvegicus
2.7.1.20 5.5
-
 0.1 mM adenosine Rattus norvegicus
2.7.1.20 5.7
-
 0.01 mM adenosine Rattus norvegicus
2.7.1.20 6 8 0.0015 mM adenosine Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.20 5.1 6.1 about half-maximal activity at pH 5.1 and pH 6.1, 0.1 mM adenosine Rattus norvegicus
2.7.1.20 5.2 6.7 about half-maximal activity at pH 5.2 and pH 6.7, 0.01 mM adenosine Rattus norvegicus
2.7.1.20 5.3 9 about half-maximal activity at pH 5.3 and about 90% of maximal activity at pH 9.0, 0.0015 mM adenosine Rattus norvegicus