Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rotllan, P.; Miras Portugal, M.T.
    Adenosine kinase from bovine adrenal medulla (1985), Eur. J. Biochem., 151, 365-371.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 adenosine above 0.002 mM, at pH 7.5 Bos taurus
2.7.1.20 adenosine-5'-pentaphospho-5'-adenosine competitive with respect to MgATP2- and uncompetitive with respect to adenosine Bos taurus
2.7.1.20 adenosine-5'-tetraphospho-5'-adenosine competitive with respect to ATP and uncompetitive with respect to adenosine Bos taurus
2.7.1.20 ADP product inhibition Bos taurus
2.7.1.20 AMP product inhibition Bos taurus
2.7.1.20 ATP free form Bos taurus
2.7.1.20 Bisadenylyl polyphosphates kinetics Bos taurus
2.7.1.20 Cu2+
-
 Bos taurus
2.7.1.20 Mg2+ free ion, strong Bos taurus
2.7.1.20 additional information no inhibition by NaVO3; product inhibition study Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 additional information
-
 additional information kinetic study Bos taurus
2.7.1.20 0.00015 0.0005 adenosine pH 7.5, 37ºC Bos taurus
2.7.1.20 0.02 0.024 ATP pH 7.5, 37ºC Bos taurus
2.7.1.20 0.02 0.024 ATP ATP in form of MgATP2- Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Ba2+ activation Bos taurus
2.7.1.20 Ca2+ activation Bos taurus
2.7.1.20 Co2+ activation Bos taurus
2.7.1.20 Co2+ more efficient than Mg2+ Bos taurus
2.7.1.20 Mg2+ requirement Bos taurus
2.7.1.20 Mg2+ reactive species: MgATP2- Bos taurus
2.7.1.20 Mn2+ activation Bos taurus
2.7.1.20 Ni2+ activation Bos taurus
2.7.1.20 Ni2+ more efficient than Mg2+ Bos taurus
2.7.1.20 Zn2+ activation Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.20 42000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.20 ATP + adenosine Bos taurus key enzyme for the regulation of intracellular adenosine level ADP + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Bos taurus
-
 male calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 ammonium sulfate precipitation, gel filtration and affinity chromatography, 1600fold purification Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.20 adrenal medulla
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.20 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.1.20 -40°C, at least 1 year Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + 2'-deoxy-adenosine
-
 Bos taurus ADP + 2'-deoxy-AMP
-
 ?
2.7.1.20 ATP + adenosine specificity Bos taurus ADP + AMP
-
 ?
2.7.1.20 ATP + adenosine ATP-analogues are also substrates Bos taurus ADP + AMP
-
 ?
2.7.1.20 ATP + adenosine broad specificity for purine trinucleotides Bos taurus ADP + AMP
-
 ?
2.7.1.20 ATP + adenosine key enzyme for the regulation of intracellular adenosine level Bos taurus ADP + AMP
-
 ?
2.7.1.20 GTP + adenosine best substrate Bos taurus GDP + AMP
-
 ?
2.7.1.20 GTP + adenosine equally effective as ITP Bos taurus GDP + AMP
-
 ?
2.7.1.20 ITP + adenosine best substrate Bos taurus IDP + AMP
-
 ?
2.7.1.20 ITP + adenosine about half as effective as GTP Bos taurus IDP + AMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.20 37
-
 assay at Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.20 0.00006
-
 adenosine-5'-tetraphospho-5'-adenosine pH 7.5, 37ºC, with respect to ATP Bos taurus
2.7.1.20 0.0004
-
 adenosine-5'-tetraphospho-5'-adenosine pH 7.5, 37ºC, with respect to adenosine Bos taurus
2.7.1.20 0.0004
-
 adenosine-5'-pentaphospho-5'-adenosine pH 7.5, 37ºC, with respect to MgATP2- Bos taurus
2.7.1.20 0.002
-
 adenosine-5'-pentaphospho-5'-adenosine pH 7.5, 37ºC, with respect to adenosine Bos taurus
2.7.1.20 0.015
-
 AMP pH 7.5, 37ºC, with respect to adenosine Bos taurus
2.7.1.20 0.15
-
 AMP pH 7.5, 37ºC, with respect to MgATP2- Bos taurus
2.7.1.20 0.2
-
 ADP pH 7.5, 37ºC, with respect to adenosine and MgATP2- Bos taurus