BRENDA - Enzyme Database

Adenosine kinase from bovine adrenal medulla

Rotllan, P.; Miras Portugal, M.T.; Eur. J. Biochem. 151, 365-371 (1985)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.20
adenosine
above 0.002 mM, at pH 7.5
Bos taurus
2.7.1.20
adenosine-5'-pentaphospho-5'-adenosine
competitive with respect to MgATP2- and uncompetitive with respect to adenosine
Bos taurus
2.7.1.20
adenosine-5'-tetraphospho-5'-adenosine
competitive with respect to ATP and uncompetitive with respect to adenosine
Bos taurus
2.7.1.20
ADP
product inhibition
Bos taurus
2.7.1.20
AMP
product inhibition
Bos taurus
2.7.1.20
ATP
free form
Bos taurus
2.7.1.20
Bisadenylyl polyphosphates
kinetics
Bos taurus
2.7.1.20
Cu2+
-
Bos taurus
2.7.1.20
Mg2+
free ion, strong
Bos taurus
2.7.1.20
additional information
no inhibition by NaVO3; product inhibition study
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.20
additional information
-
additional information
kinetic study
Bos taurus
2.7.1.20
0.00015
0.0005
adenosine
pH 7.5, 37ºC
Bos taurus
2.7.1.20
0.02
0.024
MgATP2-
pH 7.5, 37ºC
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.20
Ba2+
activation
Bos taurus
2.7.1.20
Ca2+
activation
Bos taurus
2.7.1.20
Co2+
activation; more efficient than Mg2+
Bos taurus
2.7.1.20
Mg2+
reactive species: MgATP2-; requirement
Bos taurus
2.7.1.20
Mn2+
activation
Bos taurus
2.7.1.20
Ni2+
activation; more efficient than Mg2+
Bos taurus
2.7.1.20
Zn2+
activation
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.20
42000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.20
ATP + adenosine
Bos taurus
key enzyme for the regulation of intracellular adenosine level
ADP + AMP
-
Bos taurus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.20
Bos taurus
-
male calf
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.20
ammonium sulfate precipitation, gel filtration and affinity chromatography, 1600fold purification
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.20
adrenal medulla
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.20
additional information
-
-
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.20
-40°C, at least 1 year
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.20
ATP + adenosine
specificity
641086
Bos taurus
ADP + AMP
-
-
-
?
2.7.1.20
ATP + adenosine
ATP-analogues are also substrates
641086
Bos taurus
ADP + AMP
-
-
-
?
2.7.1.20
ATP + adenosine
broad specificity for purine trinucleotides
641086
Bos taurus
ADP + AMP
-
-
-
?
2.7.1.20
ATP + adenosine
key enzyme for the regulation of intracellular adenosine level
641086
Bos taurus
ADP + AMP
-
641086
Bos taurus
?
2.7.1.20
GTP + adenosine
best substrate
641086
Bos taurus
GDP + AMP
-
641086
Bos taurus
?
2.7.1.20
GTP + adenosine
equally effective as ITP
641086
Bos taurus
GDP + AMP
-
641086
Bos taurus
?
2.7.1.20
ITP + adenosine
best substrate
641086
Bos taurus
IDP + AMP
-
641086
Bos taurus
?
2.7.1.20
ITP + adenosine
about half as effective as GTP
641086
Bos taurus
IDP + AMP
-
641086
Bos taurus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.20
37
-
assay at
Bos taurus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.20
0.00006
-
adenosine-5'-tetraphospho-5'-adenosine
pH 7.5, 37ºC, with respect to ATP
Bos taurus
2.7.1.20
0.0004
-
adenosine-5'-pentaphospho-5'-adenosine
pH 7.5, 37ºC, with respect to MgATP2-
Bos taurus
2.7.1.20
0.0004
-
adenosine-5'-tetraphospho-5'-adenosine
pH 7.5, 37ºC, with respect to adenosine
Bos taurus
2.7.1.20
0.002
-
adenosine-5'-pentaphospho-5'-adenosine
pH 7.5, 37ºC, with respect to adenosine
Bos taurus
2.7.1.20
0.015
-
AMP
pH 7.5, 37ºC, with respect to adenosine
Bos taurus
2.7.1.20
0.15
-
AMP
pH 7.5, 37ºC, with respect to MgATP2-
Bos taurus
2.7.1.20
0.2
-
ADP
pH 7.5, 37ºC, with respect to adenosine and MgATP2-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.20
adenosine
above 0.002 mM, at pH 7.5
Bos taurus
2.7.1.20
adenosine-5'-pentaphospho-5'-adenosine
competitive with respect to MgATP2- and uncompetitive with respect to adenosine
Bos taurus
2.7.1.20
adenosine-5'-tetraphospho-5'-adenosine
competitive with respect to ATP and uncompetitive with respect to adenosine
Bos taurus
2.7.1.20
ADP
product inhibition
Bos taurus
2.7.1.20
AMP
product inhibition
Bos taurus
2.7.1.20
ATP
free form
Bos taurus
2.7.1.20
Bisadenylyl polyphosphates
kinetics
Bos taurus
2.7.1.20
Cu2+
-
Bos taurus
2.7.1.20
Mg2+
free ion, strong
Bos taurus
2.7.1.20
additional information
no inhibition by NaVO3; product inhibition study
Bos taurus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.20
0.00006
-
adenosine-5'-tetraphospho-5'-adenosine
pH 7.5, 37ºC, with respect to ATP
Bos taurus
2.7.1.20
0.0004
-
adenosine-5'-pentaphospho-5'-adenosine
pH 7.5, 37ºC, with respect to MgATP2-
Bos taurus
2.7.1.20
0.0004
-
adenosine-5'-tetraphospho-5'-adenosine
pH 7.5, 37ºC, with respect to adenosine
Bos taurus
2.7.1.20
0.002
-
adenosine-5'-pentaphospho-5'-adenosine
pH 7.5, 37ºC, with respect to adenosine
Bos taurus
2.7.1.20
0.015
-
AMP
pH 7.5, 37ºC, with respect to adenosine
Bos taurus
2.7.1.20
0.15
-
AMP
pH 7.5, 37ºC, with respect to MgATP2-
Bos taurus
2.7.1.20
0.2
-
ADP
pH 7.5, 37ºC, with respect to adenosine and MgATP2-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.20
additional information
-
additional information
kinetic study
Bos taurus
2.7.1.20
0.00015
0.0005
adenosine
pH 7.5, 37ºC
Bos taurus
2.7.1.20
0.02
0.024
MgATP2-
pH 7.5, 37ºC
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.20
Ba2+
activation
Bos taurus
2.7.1.20
Ca2+
activation
Bos taurus
2.7.1.20
Co2+
activation; more efficient than Mg2+
Bos taurus
2.7.1.20
Mg2+
reactive species: MgATP2-; requirement
Bos taurus
2.7.1.20
Mn2+
activation
Bos taurus
2.7.1.20
Ni2+
activation; more efficient than Mg2+
Bos taurus
2.7.1.20
Zn2+
activation
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.20
42000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.20
ATP + adenosine
Bos taurus
key enzyme for the regulation of intracellular adenosine level
ADP + AMP
-
Bos taurus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.20
ammonium sulfate precipitation, gel filtration and affinity chromatography, 1600fold purification
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.20
adrenal medulla
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.20
additional information
-
-
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.20
-40°C, at least 1 year
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.20
ATP + adenosine
specificity
641086
Bos taurus
ADP + AMP
-
-
-
?
2.7.1.20
ATP + adenosine
ATP-analogues are also substrates
641086
Bos taurus
ADP + AMP
-
-
-
?
2.7.1.20
ATP + adenosine
broad specificity for purine trinucleotides
641086
Bos taurus
ADP + AMP
-
-
-
?
2.7.1.20
ATP + adenosine
key enzyme for the regulation of intracellular adenosine level
641086
Bos taurus
ADP + AMP
-
641086
Bos taurus
?
2.7.1.20
GTP + adenosine
best substrate
641086
Bos taurus
GDP + AMP
-
641086
Bos taurus
?
2.7.1.20
GTP + adenosine
equally effective as ITP
641086
Bos taurus
GDP + AMP
-
641086
Bos taurus
?
2.7.1.20
ITP + adenosine
best substrate
641086
Bos taurus
IDP + AMP
-
641086
Bos taurus
?
2.7.1.20
ITP + adenosine
about half as effective as GTP
641086
Bos taurus
IDP + AMP
-
641086
Bos taurus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.20
37
-
assay at
Bos taurus