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Literature summary extracted from

  • Yamada, Y.; Goto, H.; Ogasawara, N.
    Adenosine kinase from human liver (1981), Biochim. Biophys. Acta, 660, 36-43.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.20 stable when thawed and refrozen Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 adenosine substrate inhibition above 0.0005 mM Homo sapiens
2.7.1.20 Cu2+
-
Homo sapiens
2.7.1.20 EDTA
-
Homo sapiens
2.7.1.20 Mg2+ free ion, strong Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 0.00015 0.0005 adenosine pH 7.5, 30ºC Homo sapiens
2.7.1.20 0.006
-
ATP pH 7.5, 30ºC, with deoxyadenosine as substrate Homo sapiens
2.7.1.20 0.006
-
ATP ATP in form of MgATP2- Homo sapiens
2.7.1.20 0.073 0.0755 ATP ATP/Mg2+ ratio: 1 ton 2 Homo sapiens
2.7.1.20 0.073 0.0755 ATP ATP in form of MgATP2- Homo sapiens
2.7.1.20 0.36 0.67 deoxyadenosine
-
Homo sapiens
2.7.1.20 3.7
-
ribavirin
-
Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Ba2+ no activation Homo sapiens
2.7.1.20 Ca2+ can partially substitute for Mg2+ Homo sapiens
2.7.1.20 Ca2+ activation Homo sapiens
2.7.1.20 Co2+ activation Homo sapiens
2.7.1.20 Co2+ more efficient than Mg2+ Homo sapiens
2.7.1.20 Fe2+ can partially substitute for Mg2+ Homo sapiens
2.7.1.20 Fe2+ activation Homo sapiens
2.7.1.20 Mg2+ requirement Homo sapiens
2.7.1.20 Mg2+ reactive species: MgATP2- Homo sapiens
2.7.1.20 Mn2+ can partially substitute for Mg2+ Homo sapiens
2.7.1.20 Mn2+ activation Homo sapiens
2.7.1.20 additional information no activation by Zn2+ and Ba2+ Homo sapiens
2.7.1.20 additional information no activation by Cr2+ Homo sapiens
2.7.1.20 Ni2+ can partially substitute for Mg2+ Homo sapiens
2.7.1.20 Zn2+ no activation Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.20 additional information
-
amino acid composition of different species Homo sapiens
2.7.1.20 38000
-
gel filtration Homo sapiens
2.7.1.20 40000
-
gel filtration and SDS-PAGE Homo sapiens
2.7.1.20 41000
-
electrophoresis in 7.5% polyacrylamide gel in the presence of SDS Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 ammonium sulfate fractionation, affinity chromatography, gel filtration and chromatography on DEAE-cellulose Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.20 liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.20 6.3
-
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.1.20 -70°C, at least 1 year Homo sapiens
2.7.1.20 4ºC, dithiothreitol or high concentration of salt, stable. Rapid loss of activity at 4ºC with low concentration of salt, recovered when redialyzed against dithiothreitol Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + 2'-deoxy-adenosine
-
Homo sapiens ?
-
?
2.7.1.20 ATP + 2'-deoxy-adenosine
-
Homo sapiens ADP + 2'-deoxy-AMP
-
?
2.7.1.20 ATP + adenosine specificity Homo sapiens ADP + AMP
-
?
2.7.1.20 ATP + adenosine pyrimidine nucleoside triphosphates are less efficient than purine derivatives, deoxyribonucleotides less efficient than ribonucleotides Homo sapiens ADP + AMP
-
?
2.7.1.20 ATP + ribavirin
-
Homo sapiens ADP + ribavirin 5'-phosphate
-
?
2.7.1.20 GTP + adenosine best substrate Homo sapiens GDP + AMP
-
?
2.7.1.20 GTP + adenosine more effective than ATP Homo sapiens GDP + AMP
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.20 monomer 1 * 41000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.20 30
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.20 5.5
-
sharp, adenosine as substrate Homo sapiens
2.7.1.20 7.5 8.5 broad, deoxyadenosine as substrate Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.20 5 9.3 about half-maximal activity at pH 5 and pH 9.3, about 80% of maximal activity at pH 6.7, adenosine as substrate Homo sapiens
2.7.1.20 6.5 9.3 about half-maximal activity at pH 6.5 and pH 9.3, deoxyadenosine as substrate Homo sapiens