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Literature summary extracted from

  • Hawkins, C.F.; Bagnara, A.S.
    Adenosine kinase from human erythrocytes: kinetic studies and characterization of adenosine binding sites (1987), Biochemistry, 26, 1982-1987.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.20 AMP activation below 5 mM, inhibits above 5 mM Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 adenosine pH and Mg2+ dependent, most severe at pH 7.4, less inhibition at pH 8.0 and no inhibition at pH 6.5 Homo sapiens
2.7.1.20 AMP product inhibition, above 5 mM Homo sapiens
2.7.1.20 DTNB substrates protect Homo sapiens
2.7.1.20 MgADP- product inhibition, linear, noncompetitive with respect to MgATP2- and adenosine Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Mg2+ requirement Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 chromatography on CM-cellulose, chromatography on DEAE-Sephacel and affinity chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.20 ATP + adenosine = ADP + AMP mechanism Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.20 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.20 1.6
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + adenosine
-
 Homo sapiens ADP + AMP
-
 ?
2.7.1.20 GTP + adenosine best substrate Homo sapiens GDP + AMP
-
 ?
2.7.1.20 GTP + adenosine more effective than ATP Homo sapiens GDP + AMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.20 37
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.20 0.0059 0.045 adenosine pH 7.4, 37ºC, at variable AMP and Mg2+ concentrations Homo sapiens
2.7.1.20 0.03 0.534 MgADP- pH 8.0, 37ºC, at variable MgATP2- concentrations Homo sapiens
2.7.1.20 0.051 0.238 adenosine pH 8.0, 37ºC, at variable AMP and Mg2+ concentrations Homo sapiens
2.7.1.20 0.362 0.428 MgADP- pH 8.0, 37ºC, at variable adenosine concentrations Homo sapiens
2.7.1.20 1.84
-
 AMP pH 8.0, 37ºC, at variable adenosine concentrations Homo sapiens