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Literature summary extracted from

  • Chen, C.M.; Eckert, R.L.
    Phosphorylation of cytokinin by adenosine kinase from wheat germ (1977), Plant Physiol., 59, 443-447.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.20 ATP required for phosphorylation of adenosine and N6-delta2-isopentenyl-adenosine Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 0.0087
-
 adenosine pH 7.0, 37ºC Triticum aestivum
2.7.1.20 0.031
-
 N6-delta2-isopentenyl-adenosine pH 7.0, 37ºC Triticum aestivum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Mg2+ requirement Triticum aestivum
2.7.1.20 Mg2+ requirement Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Nicotiana tabacum
-
 tobacco
-
2.7.1.20 Triticum aestivum
-
 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 partial Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.20 callus
-
 Nicotiana tabacum
-
2.7.1.20 germ
-
 Triticum aestivum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + adenosine
-
 Nicotiana tabacum ADP + AMP
-
 ?
2.7.1.20 ATP + adenosine
-
 Triticum aestivum ADP + AMP 55% of AMP formed ?
2.7.1.20 ATP + ADP
-
 Triticum aestivum ADP + ATP traces of ATP formed ?
2.7.1.20 ATP + AMP
-
 Triticum aestivum ADP + ADP 45% of ADP formed ?
2.7.1.20 ATP + N6-delta2-isopentenyl-adenosine
-
 Triticum aestivum ADP + N6-delta2-isopentenyl-adenosine 5'-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.20 37
-
 assay at Triticum aestivum
2.7.1.20 37
-
 assay at Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.20 6.8 7.2 for adenosine and N6-delta2-isopentenyl-adenosine Triticum aestivum