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Literature summary extracted from

  • Xu, L.Z.; Harrison, R.W.; Weber, I.T.; Pilkis, S.J.
    Human beta-cell glucokinase. Dual role of Ser-151 in catalysis and hexose affinity (1995), J. Biol. Chem., 270, 9939-9946.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.1 N166R increased affinity for glucose and ATP by a factor of 3 Homo sapiens
2.7.1.1 N166R-S151-A lowers the KM-value for glucose by a factor of 40 and increases the KM-value for ATP Homo sapiens
2.7.1.1 S151A lowers the KM-value for glucose by a factor of 26, increases the KM-value for ATP and decreases the KM-value for mannose and fructose Homo sapiens
2.7.1.1 S151C lowers the KM-value for glucose by a factor of 2, increases the KM-value for ATP and decreases the KM-value for mannose and fructose Homo sapiens
2.7.1.1 S151G lowers the KM-value for glucose by a factor of 40, increases the KM-value for ATP and decreases the KM-value for mannose and fructose Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 D-glucose 6-phosphate
-
 Homo sapiens
2.7.1.1 N-acetylglucosamine
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.08
-
 D-mannose 30°C, pH 7.5, N166R-S151A Homo sapiens
2.7.1.1 0.12
-
 D-mannose 30°C, pH 7.5, S151G Homo sapiens
2.7.1.1 0.15
-
 D-glucose 30°C, pH 7.5, S151G and N1166R-S151A Homo sapiens
2.7.1.1 0.21
-
 D-mannose 30°C, pH 7.5, S151A Homo sapiens
2.7.1.1 0.27
-
 ATP 30°C, pH 7.5, N166R Homo sapiens
2.7.1.1 0.34
-
 D-glucose 30°C, pH 7.5, S151A Homo sapiens
2.7.1.1 0.63
-
 ATP 30°C, pH 7.5, wild-type Homo sapiens
2.7.1.1 1.52
-
 ATP 30°C, pH 7.5, N166R-S151A Homo sapiens
2.7.1.1 1.9
-
 2-deoxy-D-glucose 30°C, pH 7.5, S151A Homo sapiens
2.7.1.1 2.1
-
 D-glucose 30°C, pH 7.5, S151C and N166R Homo sapiens
2.7.1.1 2.2
-
 D-mannose 30°C, pH 7.5, N166R Homo sapiens
2.7.1.1 2.21
-
 ATP 30°C, pH 7.5, S151A Homo sapiens
2.7.1.1 2.26
-
 ATP 30°C, pH 7.5, S151G Homo sapiens
2.7.1.1 2.6
-
 ATP 30°C, pH 7.5, S151C Homo sapiens
2.7.1.1 3.74
-
 D-mannose 30°C, pH 7.5, S151C Homo sapiens
2.7.1.1 4.4
-
 D-mannose 30°C, pH 7.5, wild-type Homo sapiens
2.7.1.1 4.7
-
 D-fructose 30°C, pH 7.5, N166R-S151A Homo sapiens
2.7.1.1 6
-
 D-glucose 30°C, pH 7.5, wild-type Homo sapiens
2.7.1.1 7.7
-
 D-fructose 30°C, pH 7.5, S151G Homo sapiens
2.7.1.1 18
-
 2-deoxyglucose 30°C, pH 7.5, wild-type Homo sapiens
2.7.1.1 57.3
-
 D-fructose 30°C, pH 7.5, S151A Homo sapiens
2.7.1.1 77
-
 D-fructose 30°C, pH 7.5, N166R Homo sapiens
2.7.1.1 240
-
 D-fructose 30°C, pH 7.5, wild-type and S151C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 ATP + D-glucose Homo sapiens
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 liver
-
 Homo sapiens
-
2.7.1.1 pancreatic islet beta-cells Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 0.34
-
 S151G, D-mannose as substrate Homo sapiens
2.7.1.1 0.6
-
 S151G, D-glucose as substrate Homo sapiens
2.7.1.1 1.4
-
 N166R-S151A, D-mannose as substrate Homo sapiens
2.7.1.1 2
-
 N166R-S151A, D-glucose as substrate Homo sapiens
2.7.1.1 2.16
-
 S151C, D-mannose as substrate Homo sapiens
2.7.1.1 3.18
-
 S151G, D-fructose as substrate Homo sapiens
2.7.1.1 3.32
-
 S151A, D-mannose as substrate Homo sapiens
2.7.1.1 4.1
-
 S151A, 2-deoxy-D-glucose as substrate Homo sapiens
2.7.1.1 6
-
 S151C, D-glucose as substrate Homo sapiens
2.7.1.1 6.6
-
 S151A, D-glucose as substrate Homo sapiens
2.7.1.1 9.12
-
 S151C, D-fructose as substrate Homo sapiens
2.7.1.1 14.5
-
 N166R-S151A, D-fructose as substrate Homo sapiens
2.7.1.1 23.8
-
 S151A, D-fructose as substrate Homo sapiens
2.7.1.1 60.8
-
 N166R, D-mannose as substrate Homo sapiens
2.7.1.1 68
-
 wild-type, D-mannose and 2-deoxy-D-glucose as substrate Homo sapiens
2.7.1.1 80
-
 wild-type and N166R, D-glucose as substrate Homo sapiens
2.7.1.1 122
-
 wild-type, D-fructose as substrate Homo sapiens
2.7.1.1 310
-
 N166R, D-fructose as substrate Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose
-
 Homo sapiens ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-fructose
-
 Homo sapiens ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucose
-
 Homo sapiens ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-mannose
-
 Homo sapiens ADP + D-mannose 6-phosphate
-
 ?