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Literature summary extracted from

  • Sakuraba, H.; Mitani, Y.; Goda, S.; Kawarabayasi, Y.; Ohshima, T.
    Cloning, expression, and characterization of the first archaeal ATP-dependent glucokinase from aerobic hyperthermophilic archaeon Aeropyrum pernix (2003), J. Biochem., 133, 219-224.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.2 expression in Escherichia coli Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.2 0.054
-
 D-glucose 50°C, pH 8 Aeropyrum pernix
2.7.1.2 0.5
-
 ATP 50°C, pH 8 Aeropyrum pernix

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.2 Ca2+
-
 Aeropyrum pernix
2.7.1.2 Mg2+
-
 Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.2 35000
-
 gel filtration Aeropyrum pernix

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.2 ATP + D-glucose Aeropyrum pernix
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.2 Aeropyrum pernix
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.2 47.2
-
 recombinant protein Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.2 ATP + 2-deoxy-D-glucose no reaction with the recombinant enzyme Aeropyrum pernix ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-fructose no reaction with the recombinant enzyme Aeropyrum pernix ADP + D-fructose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucosamine no reaction with the recombinant enzyme Aeropyrum pernix ADP + D-glucosamine 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose
-
 Aeropyrum pernix ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-mannose no reaction with the recombinant enzyme Aeropyrum pernix ADP + D-mannose 6-phosphate
-
 ?
2.7.1.2 GTP + D-glucose no reaction with the recombinant enzyme Aeropyrum pernix GDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ITP + D-glucose
-
 Aeropyrum pernix IDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 UTP + D-glucose no reaction with the recombinant enzyme Aeropyrum pernix UDP + D-glucose 6-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.2 70
-
 full activity after 10 min Aeropyrum pernix
2.7.1.2 100
-
 retains 65% of activity after 10 min Aeropyrum pernix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.2 8
-
-
 Aeropyrum pernix