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Literature summary extracted from

  • Klein, D.P.; Charles, A.M.
    Purification and physical properties of glucokinase from Thiobacillus versutus (A2) (1986), Can. J. Microbiol., 32, 937-941.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.2 glucose or DTT protects against thermal inactivation at 25°C Paracoccus versutus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.2 N-acetylglucosamine weak Paracoccus versutus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.2 0.78
-
 ATP
-
 Paracoccus versutus
2.7.1.2 0.86
-
 D-glucose
-
 Paracoccus versutus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.2 Cd2+ can partially replace Mn2+ in activation Paracoccus versutus
2.7.1.2 Co2+ 38.5% of the activity with Mn2+ Paracoccus versutus
2.7.1.2 Mg2+ Km: 0.27 mM Paracoccus versutus
2.7.1.2 Mg2+ absolute requirement for divalent cation, 78% of the activity with Mn2+ Paracoccus versutus
2.7.1.2 Mn2+ absolute requirement for divalent cation, Mn2+ most effective Paracoccus versutus
2.7.1.2 Ni2+ can partially replace Mn2+ in activation Paracoccus versutus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.2 67500
-
 density gradient centrifugation Paracoccus versutus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.2 ATP + D-glucose Paracoccus versutus
-
 ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose Paracoccus versutus A2
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.2 Paracoccus versutus
-
 A2
-
2.7.1.2 Paracoccus versutus A2
-
 A2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.2
-
 Paracoccus versutus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.2 74.9
-
-
 Paracoccus versutus

Storage Stability

EC Number Storage Stability Organism
2.7.1.2 -20°C, stable for 4 weeks Paracoccus versutus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.2 ATP + D-glucose
-
 Paracoccus versutus ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose ATP is the most efficient phosphoryl donor Paracoccus versutus ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose
-
 Paracoccus versutus A2 ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose ATP is the most efficient phosphoryl donor Paracoccus versutus A2 ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 CTP + D-glucose
-
 Paracoccus versutus CDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 CTP + D-glucose
-
 Paracoccus versutus A2 CDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ITP + D-glucose 30% of the activity with ATP Paracoccus versutus IDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ITP + D-glucose 30% of the activity with ATP Paracoccus versutus A2 IDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 additional information less than 10% of the activity with ATP: TTP Paracoccus versutus ?
-
 ?
2.7.1.2 additional information less than 10% of the activity with ATP: TTP Paracoccus versutus A2 ?
-
 ?
2.7.1.2 UTP + D-glucose 12% of the activity with ATP Paracoccus versutus UDP + D-glucose 6-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.2 20 25
-
 Paracoccus versutus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.2 additional information
-
 glucose or DTT protects against thermal inactivation at 25°C Paracoccus versutus
2.7.1.2 25
-
 48 h, with glucose and DTT stable, unstable without Paracoccus versutus
2.7.1.2 55
-
 1 min, complete loss of activity Paracoccus versutus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.2 7.8
-
-
 Paracoccus versutus