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Literature summary extracted from

  • Porter, V.; Chassy, B.M.
    Glucokinase from Streptococcus mutans (1982), Methods Enzymol., 90, 25-30.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.2 rapid loss of activity in absence of DTT Streptococcus mutans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.2 6-Amino-6-deoxy-D-glucose noncompetitive to ATP and competitive to glucose Streptococcus mutans
2.7.1.2 ADP noncompetitive to ATP and uncompetitive to glucose Streptococcus mutans
2.7.1.2 beta,gamma-methyleneadenosine 5'-thiophosphate noncompetitive to ATP and uncompetitive to glucose Streptococcus mutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.2 0.21
-
 ATP
-
 Streptococcus mutans
2.7.1.2 0.61
-
 D-glucose
-
 Streptococcus mutans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.2 Ca2+ 34% of the activation with Mg2+ Streptococcus mutans
2.7.1.2 Co2+ more efficient than Mg2+ in activation, relative rate 264% Streptococcus mutans
2.7.1.2 Fe2+ 48% of the activation with Mg2+ Streptococcus mutans
2.7.1.2 Mg2+ less efficient in activation than Mn2+ or Co2+ Streptococcus mutans
2.7.1.2 Mn2+ more efficient than Mg2+ in activation, relative rate 173% Streptococcus mutans
2.7.1.2 Sr2+ 11% of the activation with Mg2+ Streptococcus mutans
2.7.1.2 Zn2+ 56% of the activation with Mg2+ Streptococcus mutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.2 ATP + D-glucose Streptococcus mutans
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.2 Streptococcus mutans
-
 OMZ70, ATCC 33535
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.2
-
 Streptococcus mutans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.2 198
-
-
 Streptococcus mutans

Storage Stability

EC Number Storage Stability Organism
2.7.1.2 -20°C, 1 mM DTT, 20-30% glycerol, indefinitely stable Streptococcus mutans
2.7.1.2 room temperature, 1 mM DTT, 5 mM MgCl2, stable for 1 week , 4°C, suspended precipitate in a 60% saturated solution of ammonium sulfate, stable for at least 3 months Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.2 ATP + D-glucose
-
 Streptococcus mutans ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose highly specific for D-glucose Streptococcus mutans ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 CTP + D-glucose less than 10% of the activity with ATP Streptococcus mutans CDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 GTP + D-glucose less than 10% of ATP Streptococcus mutans GDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ITP + D-glucose less than 10% of the activity with ATP Streptococcus mutans IDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 additional information less than 10% of the activity with ATP: TTP Streptococcus mutans ?
-
 ?
2.7.1.2 UTP + D-glucose less than 10% of the activity with ATP Streptococcus mutans UDP + D-glucose 6-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.2 7.5 9.5
-
 Streptococcus mutans