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Literature summary extracted from

  • Porter, E.V.; Chassy, B.M.; Holmlund, C.E.
    Purification and kinetic characterization of a specific glucokinase from Streptococcus mutans OMZ70 cells (1982), Biochim. Biophys. Acta, 709, 178-186.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.2 ADP noncompetitive to ATP and uncompetitive to glucose Streptococcus mutans
2.7.1.2 CTP in presence of ATP Streptococcus mutans
2.7.1.2 D-glucose 6-phosphate
-
 Streptococcus mutans
2.7.1.2 GTP in presence of ATP Streptococcus mutans
2.7.1.2 ITP in presence of ATP Streptococcus mutans
2.7.1.2 TTP in presence of ATP Streptococcus mutans
2.7.1.2 UTP in presence of ATP Streptococcus mutans
2.7.1.2 XTP in presence of ATP Streptococcus mutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.2 0.21
-
 ATP 22°C, pH 7.5 Streptococcus mutans
2.7.1.2 0.61
-
 D-glucose 22°C, pH 7.5 Streptococcus mutans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.2 Ca2+ 34% of the activation with Mg2+ Streptococcus mutans
2.7.1.2 Co2+ more efficient than Mg2+ in activation, relative rate 264% Streptococcus mutans
2.7.1.2 Fe2+ 48% of the activation with Mg2+ Streptococcus mutans
2.7.1.2 Mg2+ less efficient in activation than Mn2+ or Co2+ Streptococcus mutans
2.7.1.2 Mn2+ more efficient than Mg2+ in activation, relative rate 173% Streptococcus mutans
2.7.1.2 Zn2+ 56% of the activation with Mg2+ Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.2 24000
-
 alpha2, 2 * 24000, SDS-PAGE Streptococcus mutans
2.7.1.2 41000
-
 gel filtration Streptococcus mutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.2 ATP + D-glucose Streptococcus mutans
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.2 Streptococcus mutans
-
 OMZ70, ATCC 33535
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.2
-
 Streptococcus mutans

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.2 ATP + D-glucose = ADP + D-glucose 6-phosphate ordered mechanism in which glucose binds first and glucose 6-phosphate dissociates last Streptococcus mutans
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.2 198
-
-
 Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.2 ATP + D-glucose
-
 Streptococcus mutans ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose highly specific for D-glucose Streptococcus mutans ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 CTP + D-glucose less than 10% of the activity with ATP Streptococcus mutans CDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 GTP + D-glucose less than 10% of ATP Streptococcus mutans GDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ITP + D-glucose less than 10% of the activity with ATP Streptococcus mutans IDP + D-glucose 6-phosphate
-
 ?
2.7.1.2 additional information less than 10% of the activity with ATP: TTP Streptococcus mutans ?
-
 ?
2.7.1.2 UTP + D-glucose less than 10% of the activity with ATP Streptococcus mutans UDP + D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.2 homodimer alpha2, 2 * 24000, SDS-PAGE Streptococcus mutans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.2 22
-
 assay at Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.2 7.5 9.5
-
 Streptococcus mutans