Literature summary extracted from

  • Runquist, J.A.; Harrison, D.H.; Miziorko, H.M.
    Functional evaluation of invariant arginines situated in the mobile lid domain of phosphoribulokinase (1998), Biochemistry, 37, 1221-1226.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.19 wild-type and mutants Cereibacter sphaeroides

Engineering

EC Number Protein Variants Comment Organism
2.7.1.19 R168Q 300fold decrease in catalytic efficiency, 50fold increased Km for D-ribulose 5-phosphate Cereibacter sphaeroides
2.7.1.19 R173Q 100fold increased Km for D-ribulose 5-phosphate Cereibacter sphaeroides
2.7.1.19 R186Q decreased Km for D-ribulose 5-phosphate Cereibacter sphaeroides
2.7.1.19 R187Q increased Km for D-ribulose 5-phosphate Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.19 0.55
-
D-ribulose 5-phosphate R187Q mutant, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 4.6
-
D-ribulose 5-phosphate R168Q mutant, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 0.096
-
D-ribulose 5-phosphate wild-type enzyme, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 0.048
-
D-ribulose 5-phosphate R186Q mutant, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 10.5
-
D-ribulose 5-phosphate R173Q mutant, pH 8, 30°C Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.19 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.19
-
Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP
-
Cereibacter sphaeroides D-ribulose 1,5-diphosphate + ADP
-
?
2.7.1.19 D-ribulose 5-phosphate + trinitrophenyl-ATP
-
Cereibacter sphaeroides D-ribulose 1,5-diphosphate + trinitrophenyl-ADP
-
?