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Literature summary extracted from

  • Runquist, J.A.; Harrison, D.H.T.; Miziorko, H.M.
    Rhodobacter sphaeroides phosphoribulokinase: identification of lysine-165 as a catalytic residue and evaluation of the contributions of invariant basic amino acids to ribulose 5-phosphate binding (1999), Biochemistry, 38, 13999-14005.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.19 wild-type and mutants Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.19 K165C reduced catalytic activity Cereibacter sphaeroides
2.7.1.19 K165M reduced catalytic activity, insensitive to inactivation by pyridoxal 5'-phosphate Cereibacter sphaeroides
2.7.1.19 K53M sensitive to inactivation by pyridoxal 5'-phosphate Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.19 6-phospho-D-gluconate competitive with respect to D-ribulose 5-phosphate Cereibacter sphaeroides
2.7.1.19 pyridoxal 5'-phosphate inactivation is protected by ATP in combination with NADH Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.19 0.096
-
 D-ribulose 5-phosphate wild-type enzyme, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 10.3
-
 D-ribulose 5-phosphate K165M mutant, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 11
-
 D-ribulose 5-phosphate K165C mutant, pH 8, 30°C Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.19 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.19 wild-type and mutant enzymes Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP
-
 Cereibacter sphaeroides D-ribulose 1,5-diphosphate + ADP
-
 ?
2.7.1.19 D-ribulose 5-phosphate + trinitrophenyl-ATP spectroscopically active ATP-analog Cereibacter sphaeroides D-ribulose 1,5-diphosphate + trinitrophenyl-ADP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.19 30
-
 assay at Cereibacter sphaeroides

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.19 0.222
-
 6-phospho-D-gluconate wild-type enzyme, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 0.415
-
 6-phospho-D-gluconate R168Q mutant, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 1.15
-
 6-phospho-D-gluconate R173Q mutant, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 2.81
-
 6-phospho-D-gluconate H45N mutant, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 11
-
 6-phospho-D-gluconate K165M mutant, pH 8, 30°C Cereibacter sphaeroides
2.7.1.19 19.8
-
 6-phospho-D-gluconate R49Q mutant, pH 8, 30°C Cereibacter sphaeroides