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Literature summary extracted from

  • Abdelal, A.T.H.; Schlegel, H.G.
    Purification and regulatory properties of phosphoribulokinase from Hydrogenomonas eutropha H 16 (1974), Biochem. J., 139, 481-489.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.19 NADH 1 mM Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.19 AMP 50% inhibition at 0.08 mM Cupriavidus necator
2.7.1.19 phosphoenolpyruvate
-
 Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.19 additional information
-
 additional information kinetics Cupriavidus necator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.19 Mg2+ required Cupriavidus necator
2.7.1.19 Mg2+ Km: ca. 0.5 mM Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.19 230000
-
 gel filtration Cupriavidus necator
2.7.1.19 237000
-
-
 Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP Cupriavidus necator
-
 D-ribulose 1,5-diphosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.19 Cupriavidus necator
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.19 286fold Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.19 88.9
-
-
 Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
2.7.1.19 4°C, 0.02 M triethanolamine-HCl buffer, pH 7.6, 0.5 mM EDTA, 0.5 mM dithiothreitol, 4 months Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.19 D-ribulose 5-phosphate + ATP
-
 Cupriavidus necator D-ribulose 1,5-diphosphate + ADP
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.19 9
-
-
 Cupriavidus necator

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.19 8.2
-
 50% of maximal activity Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.19 NADH activation Cupriavidus necator