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Literature summary extracted from
- Chlamydomonas reinhardtii phosphoribulokinase. Sequence, purification, and kinetics (1990), Plant Physiol., 93, 188-193.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.1.19 | 0.056 | - |
D-ribulose 5-phosphate | pH 7.8, 25°C | Chlamydomonas reinhardtii |  |
| 2.7.1.19 | 0.062 | - |
ATP | pH 7.8, 25°C | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.19 | 38500 | - |
x * 38500, calculated from sequence of cDNA, SDS-PAGE | Chlamydomonas reinhardtii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.19 | D-ribulose 5-phosphate + ATP | Chlamydomonas reinhardtii | - |
D-ribulose 1,5-diphosphate + ADP | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.1.19 | Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.1.19 | homogeneity | Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.19 | 410 | - |
- |
Chlamydomonas reinhardtii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.7.1.19 | -80°C, 100 mM Tris buffer, pH 8.0, 0.5 mM dithiothreitol, 10% v/v glycerol | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.19 | D-ribulose 5-phosphate + ATP | - |
Chlamydomonas reinhardtii | D-ribulose 1,5-diphosphate + ADP | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.1.19 | ? | x * 38500, calculated from sequence of cDNA, SDS-PAGE | Chlamydomonas reinhardtii |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 2.7.1.19 | Chlamydomonas reinhardtii | calculated from amino acid sequence | - |
5.75 |
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