EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.15 | Li+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | additional information | 2-deoxyribose-5-phosphate and ADP at concentrations up to 5.4 and 10 mM do not inhibit the reaction with nonsaturating levels of substrates | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | Na+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.15 | 0.1 | - |
2-deoxy-D-ribose | 25°C, pH 7.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | 0.5 | - |
ATP | 25°C, pH 7.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | 2 | - |
D-ribose | 25°C, pH 7.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | 33.3 | - |
2-deoxy-D-ribitol | 25°C, pH 7.5 | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.15 | Cd2+ | can replace Mg2+ in activation, optimal concentration: 5 mM, 68% of the activity with Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | Co2+ | can replace Mg2+ in activation, optimal concentration: 5 mM, 87% of the activity with Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | Cs+ | optimal activity obtained in presence of 0.5 M NH4+ or Cs+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | Cu2+ | can replace Mg2+ in activation, optimal concentration: 10 mM, 20% of the activity with Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | K+ | stimulates | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | Mg2+ | absolute requirement for divalent cations, best satisfied by Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | Mg2+ | the change in the ratio from Mg2+ to ATP from 1 : 1 to 2 : 1 gives optimal activity | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | Mn2+ | can replace Mg2+ in activation | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | Mn2+ | optimal concentration 5 mM, 61% of the activity with Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | NH4+ | optimal activity obtained in presence of 0.5 M NH4+ or Cs+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | Ni2+ | can replace Mg2+, optimal concentration: 25-30 mM, 31% of the activity with Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | Rb+ | stimulates | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.1.15 | Zn2+ | can replace Mg2+ in activation, optimal concentration: 1 mM, 27% of the activity with Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.15 | 33500 | - |
alpha2, 2 * 33500, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.1.15 | 67800 | - |
high-speed sedimentation equilibrium method | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.15 | ATP + 2-deoxy-D-ribose | Salmonella enterica subsp. enterica serovar Typhimurium | first step in the inducible fermentation pathway for this sugar | ADP + 2-deoxy-D-ribose 5-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.15 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
deoxyribose-grown cells | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.15 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.15 | 70 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.15 | ATP + 2-deoxy-D-ribitol | 41% of the activity with 2-deoxy-D-ribose | Salmonella enterica subsp. enterica serovar Typhimurium | ADP + 2-deoxy-D-ribitol 5-phosphate | - |
? | |
2.7.1.15 | ATP + 2-deoxy-D-ribose | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + 2-deoxy-D-ribose 5-phosphate | - |
? | |
2.7.1.15 | ATP + 2-deoxy-D-ribose | first step in the inducible fermentation pathway for this sugar | Salmonella enterica subsp. enterica serovar Typhimurium | ADP + 2-deoxy-D-ribose 5-phosphate | - |
? | |
2.7.1.15 | ATP + D-ribose | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + D-ribose 5-phosphate | - |
? | |
2.7.1.15 | CTP + D-ribose | 18% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | CDP + D-ribose 5-phosphate | - |
? | |
2.7.1.15 | dATP + D-ribose | 108% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | dADP + D-ribose 5-phosphate | - |
? | |
2.7.1.15 | dCTP + D-ribose | 25% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | dCDP + D-ribose 5-phosphate | - |
? | |
2.7.1.15 | dGTP + D-ribose | 50% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | dGDP + D-ribose 5-phosphate | - |
? | |
2.7.1.15 | GTP + D-ribose | 32% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | GDP + D-ribose 5-phosphate | - |
? | |
2.7.1.15 | ITP + D-ribose | 37% of the activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | IDP + D-ribose 5-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.15 | dimer | alpha2, 2 * 33500, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.15 | 25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.15 | 6.8 | 7.2 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |