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Literature summary extracted from

  • Bissett, D.L.; Anderson, R.L.
    Lactose and D-galactose metabolism in Staphylococcus aureus. III: Purification and properties of D-tagatose-6-phosphate kinase (1980), J. Biol. Chem., 255, 8745-8749.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.144 NH4+ 33.3 mM activates Staphylococcus aureus

General Stability

EC Number General Stability Organism
2.7.1.144 2-mercaptoethanol stabilizes during purification Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.144 Li+ 33.3 mM, 65% inhibition Staphylococcus aureus
2.7.1.144 Na+ 33.3 mM, 31% inhibition Staphylococcus aureus
2.7.1.144 NEM
-
 Staphylococcus aureus
2.7.1.144 phosphate at high concentrations Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.144 0.016
-
 D-tagatose 6-phosphate
-
 Staphylococcus aureus
2.7.1.144 0.15
-
 D-fructose 6-phosphate
-
 Staphylococcus aureus
2.7.1.144 0.16
-
 ATP
-
 Staphylococcus aureus
2.7.1.144 0.17
-
 ITP
-
 Staphylococcus aureus
2.7.1.144 0.4
-
 GTP
-
 Staphylococcus aureus
2.7.1.144 0.9
-
 UTP
-
 Staphylococcus aureus
2.7.1.144 18
-
 CTP
-
 Staphylococcus aureus
2.7.1.144 20
-
 TTP
-
 Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.144 Cd2+ 8% of the activation with Mg2+, at 1.3 mM Staphylococcus aureus
2.7.1.144 Co2+ 54% of the activation with Mg2+, at 1.3 mM Staphylococcus aureus
2.7.1.144 K+ 33.3 mM activates Staphylococcus aureus
2.7.1.144 Mg2+ absolute requirement for a divalent cation, a Mg2+ : ATP ratio of 2.0-2.5 is required for maximal activity Staphylococcus aureus
2.7.1.144 Mn2+ 27% of the activation with Mg2+, at 1.3 mM Staphylococcus aureus
2.7.1.144 Ni2+ 15% of the activation with Mg2+, at 1.3 mM Staphylococcus aureus
2.7.1.144 Rb+ 33.3 mM activates Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.144 52000
-
 alpha2, 2 * 52000, SDS-PAGE Staphylococcus aureus
2.7.1.144 82000
-
 gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.144 ATP + D-tagatose 6-phosphate Staphylococcus aureus inducible by D-galactose ADP + D-tagatose 1,6-bisphosphate
-
 ?
2.7.1.144 ATP + D-tagatose 6-phosphate Staphylococcus aureus involved in the catabolism of lactose and D-galactose ADP + D-tagatose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.144 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.144
-
 Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.144 17.5
-
-
 Staphylococcus aureus

Storage Stability

EC Number Storage Stability Organism
2.7.1.144 -20°C, 20 mM potassium phosphate buffer, pH 7.5, 20% v/v glycerol, 0.2% v/v 2-mercaptoethanol, stable for several months Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.144 ATP + D-fructose 6-phosphate
-
 Staphylococcus aureus ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.144 ATP + D-tagatose 6-phosphate
-
 Staphylococcus aureus ADP + D-tagatose 1,6-bisphosphate
-
 ?
2.7.1.144 ATP + D-tagatose 6-phosphate inducible by D-galactose Staphylococcus aureus ADP + D-tagatose 1,6-bisphosphate
-
 ?
2.7.1.144 ATP + D-tagatose 6-phosphate involved in the catabolism of lactose and D-galactose Staphylococcus aureus ADP + D-tagatose 1,6-bisphosphate
-
 ?
2.7.1.144 CTP + D-tagatose 6-phosphate
-
 Staphylococcus aureus CDP + D-tagatose 1,6-bisphosphate
-
 ?
2.7.1.144 GTP + D-tagatose 6-phosphate
-
 Staphylococcus aureus GDP + D-tagatose 1,6-bisphosphate
-
 ?
2.7.1.144 ITP + D-tagatose 6-phosphate
-
 Staphylococcus aureus IDP + D-tagatose 1,6-bisphosphate
-
 ?
2.7.1.144 TTP + D-tagatose 6-phosphate
-
 Staphylococcus aureus TDP + D-tagatose 1,6-bisphosphate
-
 ?
2.7.1.144 UTP + D-tagatose 6-phosphate
-
 Staphylococcus aureus UDP + D-tagatose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.144 dimer alpha2, 2 * 52000, SDS-PAGE Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.144 7.5
-
 in potassium phosphate buffer Staphylococcus aureus
2.7.1.144 8
-
 in Hepes buffer Staphylococcus aureus
2.7.1.144 8.5
-
 in glycylglycine buffer, bicine buffer and Tris buffer Staphylococcus aureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.144 7.5 9.5 pH 7.5: about 75% of maximal activity, pH 9.5: about 55% of maximal activity, glycylglycine buffer Staphylococcus aureus