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Literature summary extracted from

  • Ono, F.; Nakagawa, T.; Saito, S.; Owada, Y.; Sakagami, H.; Goto, K.; Suzuki, M.; Matsuno, S.; Kondo, H.
    A novel class II phosphoinositide 3-kinase predominantly expressed in the liver and its enhanced expression during liver regeneration (1998), J. Biol. Chem., 273, 7731-7736.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.137
-
 Rattus norvegicus
2.7.1.154 expression in COS-7 cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.154 Mn2+ 3.5 mM, instead of Mg2+, almost complete inhibition Rattus norvegicus
2.7.1.154 Nonidet P-40 0.5%, almost complete inhibition Rattus norvegicus
2.7.1.154 Triton X-100 0.5%, almost complete inhibition Rattus norvegicus
2.7.1.154 Wortmannin IC50: 12 nM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.137 juxtanuclear Golgi region
-
 Rattus norvegicus
-
-
2.7.1.137 nuclear membrane at low levels Rattus norvegicus 31965
-
2.7.1.137 plasma membrane at low levels Rattus norvegicus 5886
-
2.7.1.154 juxtanuclear Golgi region at high levels Rattus norvegicus
-
-
2.7.1.154 nuclear membrane at low level Rattus norvegicus 31965
-
2.7.1.154 plasma membrane at low level Rattus norvegicus 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.154 170972
-
 x * 170972, calculation from nucleotide sequence Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.137 Rattus norvegicus O70173
-
-
2.7.1.154 Rattus norvegicus O70173 PI3-C2gamma
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.137 liver expression increases during liver regeneration after partial hepatectomy with maximal expression after the growth period, PI3K-IIgamma may function mainly in highly differentiated hepatic cells Rattus norvegicus
-
2.7.1.154 liver PI3K-IIgamma mRNA expression is confined to the liver throughout the development with much higher expression in adult liver than in fetal liver. Expression increases during liver regeneration after partial hepatectomy. PI3K-gamma may function mainly in highly differentiated hepatic cells Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.137 ATP + 1-phosphatidylinositol
-
 Rattus norvegicus ADP + phosphatidylinositol 3-phosphate
-
 ?
2.7.1.154 ATP + 1-phosphatidyl-1D-myo-inositol-4-phosphate
-
 Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
-
 ?
2.7.1.154 ATP + phosphatidylinositol
-
 Rattus norvegicus ADP + phosphatidylinositol 3-phosphate
-
 ?
2.7.1.154 additional information no activity with phosphatidylinositol 4,5-diphosphate Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.154 ? x * 170972, calculation from nucleotide sequence Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.154 0.000012
-
 IC50: 12 nM Rattus norvegicus Wortmannin