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Literature summary extracted from

  • Domin, J.; Pages, F.; Volinia, S.; Rittenhouse, S.E.; Zvelebil, M.J.; Stein, R.C.; Waterfield, M.D.
    Cloning of a human phosphoinositide 3-kinase with a C2 domain that displays reduced sensitivity to the inhibitor wortmannin (1997), Biochem. J., 326, 139-147.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.137 cloning of a human phosphoinositide 3-kinase with a C2 domain that displays reduced sensitivity to the inhibitor wortmannin Homo sapiens
2.7.1.154
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.137 Wortmannin phosphoinositide 3-kinase with a C2 domain displays reduced sensitivity to the inhibitor wortmannin Homo sapiens
2.7.1.154 LY294002 IC50: 0.019 mM, maximal inhibition at 1 mM Homo sapiens
2.7.1.154 additional information introduction of phosphatidylserine, produces an 8fold decrease in Vmax for ATP using phosphatidylinositol. Without phosphatidylserine, phosphatidylinositol 4-phosphate produces non-linear enzyme kinetics Homo sapiens
2.7.1.154 Wortmannin IC50: 420 nM, maximal inhibition at 10 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.154 0.015
-
 ATP pH 7.5, 30°C, reaction with phosphatidylinositol, without phosphatidylserine Homo sapiens
2.7.1.154 0.025
-
 phosphatidylinositol 4-phosphate pH 7.5, 30°C, in presence of phosphatidylserine Homo sapiens
2.7.1.154 0.032
-
 ATP pH 7.5, 30°C, reaction with phosphatidylinositol, in presence of phosphatidylserine Homo sapiens
2.7.1.154 0.054
-
 ATP pH 7.5, 30°C, reaction with phosphatidylinositol 4-phosphate, without phosphatidylserine Homo sapiens
2.7.1.154 0.064
-
 phosphatidylinositol pH 7.5, 30°C, in presence of phosphatidylserine Homo sapiens
2.7.1.154 0.122
-
 phosphatidylinositol pH 7.5, 30°C, without phosphatidylserine Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.154 190000
-
 x * 190000, calculation from nucleotide sequence Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.137 Homo sapiens
-
-
-
2.7.1.154 Homo sapiens
-
 PI3K-C2alpha
-
2.7.1.154 Homo sapiens PI3K-C2alpha
-
 PI3K-C2alpha
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.154 heart
-
 Homo sapiens
-
2.7.1.154 kidney mRNA is undetectable in kidney Homo sapiens
-
2.7.1.154 ovary
-
 Homo sapiens
-
2.7.1.154 placenta
-
 Homo sapiens
-
2.7.1.154 testis
-
 Homo sapiens
-
2.7.1.154 U-937 cell monocyte Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.137 ATP + 1-phosphatidylinositol
-
 Homo sapiens ADP + phosphatidylinositol 3-phosphate
-
 ?
2.7.1.137 ATP + 1-phosphatidylinositol
-
 Homo sapiens PI3K-C2alpha ADP + phosphatidylinositol 3-phosphate
-
 ?
2.7.1.154 ATP + 1-phosphatidyl-1D-myo-inositol-4-phosphate
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
-
 ?
2.7.1.154 ATP + 1-phosphatidyl-1D-myo-inositol-4-phosphate
-
 Homo sapiens PI3K-C2alpha ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
-
 ?
2.7.1.154 ATP + phosphatidylinositol
-
 Homo sapiens ADP + phosphatidylinositol 3-phosphate
-
 ?
2.7.1.154 ATP + phosphatidylinositol
-
 Homo sapiens PI3K-C2alpha ADP + phosphatidylinositol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.154 ? x * 190000, calculation from nucleotide sequence Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.154 0.00042
-
 IC50: 420 nM, maximal inhibition at 10 mM Homo sapiens Wortmannin
2.7.1.154 0.019
-
 IC50: 0.019 mM, maximal inhibition at 1 mM Homo sapiens LY294002