BRENDA - Enzyme Database

Purification and characterization of phosphoinositide 3-kinase from rat liver

Carpenter, C.L.; Duckworth, B.C.; Auger, K.R.; Cohen, B.; Schaffhausen, B.S.; Cantley, L.C.; J. Biol. Chem. 265, 19704-19711 (1990)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.137
cardiolipin
-
Rattus norvegicus
2.7.1.137
lysophosphatidic acid
-
Rattus norvegicus
2.7.1.137
phosphatidic acid
-
Rattus norvegicus
2.7.1.137
phosphatidylcholine
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.137
0.004
-
phosphatidylinositol 4,5-bisphosphate
-
Rattus norvegicus
2.7.1.137
0.009
-
phosphatidylinositol 4-phosphate
-
Rattus norvegicus
2.7.1.137
0.03
-
ATP
with phosphatidylinositol 4-phosphate or phosphatidylinositol 4,5-bisphosphate as cosubstrate
Rattus norvegicus
2.7.1.137
0.06
-
ATP
with phosphatidylinositol as cosubstrate
Rattus norvegicus
2.7.1.137
0.06
-
phosphatidylinositol
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.137
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.137
Ca2+
no effect at physiological concentrations
Rattus norvegicus
2.7.1.137
Mg2+
maximal activation at 5 mM; required
Rattus norvegicus
2.7.1.137
Mn2+
can partially replace Mg2+ in activation, about 10% of the activity with Mg2+
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.137
85000
-
1 * 110000 + 1 * 85000, SDS-PAGE
Rattus norvegicus
2.7.1.137
110000
-
1 * 110000 + 1 * 85000, SDS-PAGE
Rattus norvegicus
2.7.1.137
190000
-
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.137
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.137
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.137
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.1.137
0.058
-
production of phosphatidylinositol 3,4-diphosphate
Rattus norvegicus
2.7.1.137
0.124
-
production of phosphatidylinositol 3-phosphate
Rattus norvegicus
2.7.1.137
0.214
-
production of phosphatidylinositol 3,4,5-triphosphate
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.137
ATP + 1-phosphatidyl-1D-myo-inositol
-
640851
Rattus norvegicus
ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
-
-
-
2.7.1.137
ATP + 1-phosphatidylinositol
-
640851
Rattus norvegicus
ADP + phosphatidylinositol 3-phosphate
-
640851
Rattus norvegicus
?
2.7.1.137
ATP + phosphatidylinositol 4,5-bisphosphate
-
640851
Rattus norvegicus
ADP + phosphatidylinositol 3,4,5-trisphosphate
-
640851
Rattus norvegicus
?
2.7.1.137
ATP + phosphatidylinositol 4-phosphate
-
640851
Rattus norvegicus
ADP + phosphatidylinositol 3,4-bisphosphate
-
640851
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.137
dimer
1 * 110000 + 1 * 85000, SDS-PAGE
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.137
7
8
-
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.137
6.5
9
pH 6.5: about 80% of maximal activity, pH 9.0: about 40% of maximal activity
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.137
cardiolipin
-
Rattus norvegicus
2.7.1.137
lysophosphatidic acid
-
Rattus norvegicus
2.7.1.137
phosphatidic acid
-
Rattus norvegicus
2.7.1.137
phosphatidylcholine
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.137
0.004
-
phosphatidylinositol 4,5-bisphosphate
-
Rattus norvegicus
2.7.1.137
0.009
-
phosphatidylinositol 4-phosphate
-
Rattus norvegicus
2.7.1.137
0.03
-
ATP
with phosphatidylinositol 4-phosphate or phosphatidylinositol 4,5-bisphosphate as cosubstrate
Rattus norvegicus
2.7.1.137
0.06
-
ATP
with phosphatidylinositol as cosubstrate
Rattus norvegicus
2.7.1.137
0.06
-
phosphatidylinositol
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.137
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.137
Ca2+
no effect at physiological concentrations
Rattus norvegicus
2.7.1.137
Mg2+
maximal activation at 5 mM; required
Rattus norvegicus
2.7.1.137
Mn2+
can partially replace Mg2+ in activation, about 10% of the activity with Mg2+
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.137
85000
-
1 * 110000 + 1 * 85000, SDS-PAGE
Rattus norvegicus
2.7.1.137
110000
-
1 * 110000 + 1 * 85000, SDS-PAGE
Rattus norvegicus
2.7.1.137
190000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.137
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.137
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.1.137
0.058
-
production of phosphatidylinositol 3,4-diphosphate
Rattus norvegicus
2.7.1.137
0.124
-
production of phosphatidylinositol 3-phosphate
Rattus norvegicus
2.7.1.137
0.214
-
production of phosphatidylinositol 3,4,5-triphosphate
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.137
ATP + 1-phosphatidyl-1D-myo-inositol
-
640851
Rattus norvegicus
ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
-
-
-
2.7.1.137
ATP + 1-phosphatidylinositol
-
640851
Rattus norvegicus
ADP + phosphatidylinositol 3-phosphate
-
640851
Rattus norvegicus
?
2.7.1.137
ATP + phosphatidylinositol 4,5-bisphosphate
-
640851
Rattus norvegicus
ADP + phosphatidylinositol 3,4,5-trisphosphate
-
640851
Rattus norvegicus
?
2.7.1.137
ATP + phosphatidylinositol 4-phosphate
-
640851
Rattus norvegicus
ADP + phosphatidylinositol 3,4-bisphosphate
-
640851
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.137
dimer
1 * 110000 + 1 * 85000, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.137
7
8
-
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.137
6.5
9
pH 6.5: about 80% of maximal activity, pH 9.0: about 40% of maximal activity
Rattus norvegicus