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Literature summary extracted from

  • Luo, X.; Crawley, S.W.; Steimle, P.A.; Egelhoff, T.T.; Cote, G.P.
    Specific phosphorylation of threonine by the Dictyostelium myosin II heavy chain kinase family (2001), J. Biol. Chem., 276, 17836-17843.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.7 expression of the catalytic domain of MHCK B as a GST-fusion protein in Escherichia coli BL21(DE3) Dictyostelium discoideum
2.7.11.7 expression of the His-tagged catalytic domain of MHCK A in Escherichia coli BL21(DE3) Dictyostelium discoideum
2.7.11.7 expression of the His-tagged catalytic domain of MHCK C in Escherichia coli BL21(DE3) Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.7 0.28
-
RKKFGESEKTKTKEFL-amide pH 7.0, 25°C Dictyostelium discoideum
2.7.11.7 0.55
-
YAYDTRYRR pH 7.0, 25°C Dictyostelium discoideum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Mg2+
-
Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin II heavy chain Dictyostelium discoideum
-
ADP + myosin II heavy chain phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Dictyostelium discoideum O76739 gene MHCK C, myosin II heavy chain kinase C
-
2.7.11.7 Dictyostelium discoideum P42527 gene MHCK A, myosin II heavy chain kinase A
-
2.7.11.7 Dictyostelium discoideum P90648 gene MHCK B, myosin II heavy chain kinase B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 recombinant GST-fusion catalytic domain of MHCK B, expressed in Escherichia coli Dictyostelium discoideum
2.7.11.7 recombinant His-tagged catalytic domain of MHCK A, expressed in Escherichia coli Dictyostelium discoideum
2.7.11.7 recombinant His-tagged catalytic domain of MHCK C, expressed in Escherichia coli Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + caldesmon catalytic domain Dictyostelium discoideum ADP + caldesmon phosphate
-
?
2.7.11.7 ATP + casein catalytic domain, low activity Dictyostelium discoideum ADP + casein phosphate
-
?
2.7.11.7 ATP + myelin basic protein catalytic domain Dictyostelium discoideum ADP + myelin basic protein phosphate
-
?
2.7.11.7 ATP + myosin II heavy chain
-
Dictyostelium discoideum ADP + myosin II heavy chain phosphate
-
?
2.7.11.7 ATP + myosin II heavy chain catalytic domain Dictyostelium discoideum ADP + myosin II heavy chain phosphate
-
?
2.7.11.7 ATP + myosin II heavy chain phosphorylates primarly threonine residues Dictyostelium discoideum ADP + myosin II heavy chain phosphate
-
?
2.7.11.7 ATP + myosin regulatory light chain catalytic domain Dictyostelium discoideum ADP + myosin regulatory light chain phosphate
-
?
2.7.11.7 ATP + RKKFGESEKTKTKEFL-amide low activity Dictyostelium discoideum ?
-
?
2.7.11.7 ATP + RKKFGESEKTKTKEFL-amide catalytic domain Dictyostelium discoideum ?
-
?
2.7.11.7 ATP + RKKFGESEKTKTKEFL-amide synthetic peptide MH-3 Dictyostelium discoideum ?
-
?
2.7.11.7 ATP + YAYDTRYRR consensus sequence Dictyostelium discoideum ?
-
?
2.7.11.7 ATP + YAYDTRYRR catalytic domain of the enzyme Dictyostelium discoideum ?
-
?
2.7.11.7 additional information localization of autophosphorylation sites Dictyostelium discoideum ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.7 MHCK
-
Dictyostelium discoideum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 25
-
assay at Dictyostelium discoideum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.11.7 additional information
-
additional information
-
Dictyostelium discoideum
2.7.11.7 additional information
-
additional information catalytic domain, several substrates Dictyostelium discoideum
2.7.11.7 0.01
-
RKKFGESEKTKTKEFL-amide pH 7.0, 25°C Dictyostelium discoideum
2.7.11.7 0.55
-
RKKFGESEKTKTKEFL-amide pH 7.0, 25°C Dictyostelium discoideum
2.7.11.7 0.79
-
RKKFGESEKTKTKEFL-amide pH 7.0, 25°C Dictyostelium discoideum
2.7.11.7 14
-
YAYDTRYRR pH 7.0, 25°C Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 7
-
assay at Dictyostelium discoideum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
Dictyostelium discoideum