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Literature summary extracted from

  • Rieker, J.P.; Swanljung-Collins, H.; Montibeller, J.; Collins, J.H.
    Isolation and characterization of calmodulin-dependent myosin heavy chain kinase from intestinal brush border (1987), Methods Enzymol., 139, 105-114.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.11.7 freeze-thawing inactivates Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.7 EGTA
-
 Gallus gallus
2.7.11.7 NaCl above 0.1 M Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.7 cytosol
-
 Gallus gallus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Ca2+ requirement, Ca2+/calmodulin-dependent isozyme Gallus gallus
2.7.11.7 Mg2+ requirement Gallus gallus
2.7.11.7 Mg2+ optimal activation at 6 mM Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin heavy chain Gallus gallus
-
 ADP + myosin heavy chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Gallus gallus
-
 two myosin heavy-chain kinases: a Ca2+/calmodulin dependent and a Ca2+/calmodulin independent enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 separation of the Ca2+/calmodulin-dependent and -independent isozymes Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.7 epithelium of the intestine, brush border cells Gallus gallus
-
2.7.11.7 small intestine epithelium Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.7 0.00032
-
 partially purified enzyme Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.7.11.7 0°C, further purified Ca2+/calmodulin-dependent isozyme, complete loss of activity within 1 day Gallus gallus
2.7.11.7 0°C, partly purified Ca2+/calmodulin-dependent isozyme, at least 2 weeks Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + myosin heavy chain
-
 Gallus gallus ADP + myosin heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain phosphorylates threonine residues Gallus gallus ADP + myosin heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain best substrate: chicken intestine brush border myosin Gallus gallus ADP + myosin heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain specific for myosin heavy chains, no phosphorylation of light chains Gallus gallus ADP + myosin heavy chain phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 30
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 7 7.5
-
 Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Gallus gallus