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Literature summary extracted from

  • Hammer, J.A.; Albanesi, J.P.; Korn, E.D.
    Purification and characterization of a myosin I heavy chain kinase from Acanthamoeba castellanii (1983), J. Biol. Chem., 258, 10168-10175.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.7 additional information no activation by calmodulin, cAMP Acanthamoeba castellanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.7 Ca2+/calmodulin no inhibition Acanthamoeba castellanii
2.7.11.7 additional information strong inhibition of phosphorylation activity by increasing ionic strength Acanthamoeba castellanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.7 0.043
-
 ATP pH 7.5, 30°C Acanthamoeba castellanii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.7 cytosol
-
 Acanthamoeba castellanii 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Ca2+ not required Acanthamoeba castellanii
2.7.11.7 Mg2+ requirement Acanthamoeba castellanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.7 107000
-
 gel filtration Acanthamoeba castellanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin I heavy chain Acanthamoeba castellanii
-
 ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin II heavy chain Acanthamoeba castellanii
-
 ADP + myosin II heavy chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Acanthamoeba castellanii
-
 myosin I heavy chain kinase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 affinity chromatography using histone-resin Acanthamoeba castellanii
2.7.11.7 to near homogeneity Acanthamoeba castellanii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.7 cell culture
-
 Acanthamoeba castellanii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.7 3.03
-
 purified enzyme, substrate histone 2A, 0.067 mM Acanthamoeba castellanii
2.7.11.7 4.6
-
 purified enzyme, substrate myosin IB, 0.0024 mM Acanthamoeba castellanii

Storage Stability

EC Number Storage Stability Organism
2.7.11.7 -20°C, in 50% glycerol, 3 months stable and less than 20% loss of activity within 6 months Acanthamoeba castellanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + casein poor substrate Acanthamoeba castellanii ADP + casein phosphate
-
 ?
2.7.11.7 ATP + histone 2A
-
 Acanthamoeba castellanii ADP + histone 2A phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase intramolecular autophosphorylation Acanthamoeba castellanii ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain
-
 Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain substrate: myosin IB, phosphorylation site is Ser315 Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain phosphorylates serine-residues Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain incorporation of 0.9-1.0 mol phosphate per mol of heavy chain myosin Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain contains two myosin heavy chain kinases: one for myosin I and one for myosin II Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain substrates are heavy chains of myosin IA and IB Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin II heavy chain
-
 Acanthamoeba castellanii ADP + myosin II heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin II heavy chain contains two myosin heavy chain kinases: one for myosin I and one for myosin II Acanthamoeba castellanii ADP + myosin II heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin II heavy chain phosphorylates serine residues Acanthamoeba castellanii ADP + myosin II heavy chain phosphate
-
 ?
2.7.11.7 ATP + phosvitin no activity Acanthamoeba castellanii ADP + phosvitin phosphate
-
 ?
2.7.11.7 ATP + smooth muscle myosin light chain
-
 Acanthamoeba castellanii ADP + smooth muscle myosin light chain phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.7 monomer 1 * 107000, phosphorylated enzyme, SDS-PAGE Acanthamoeba castellanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 30
-
 assay at Acanthamoeba castellanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 7 7.5
-
 Acanthamoeba castellanii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.7 6 8.5 about half-maximal activity at pH 6.0 Acanthamoeba castellanii
2.7.11.7 6 8.5 about 80% activity at pH 6.0 Acanthamoeba castellanii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Acanthamoeba castellanii