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Literature summary extracted from

  • Ravid, S.; Spudich, J.A.
    Myosin heavy chain kinase from developed Dictyostelium cells. Purification and characterization (1989), J. Biol. Chem., 264, 15144-15150.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.7 additional information no activation by cGMP Dictyostelium discoideum
2.7.11.7 additional information no activation by calmodulin, cAMP Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.7 KCl 80% inhibition at 90 mM Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.7 0.015
-
 [myosin heavy-chain] pH 7.5, 22°C Dictyostelium discoideum
2.7.11.7 0.015
-
 [protein LMM58 heavy chain] pH 7.5, 22°C Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.7 membrane associated Dictyostelium discoideum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Ca2+ not required Dictyostelium discoideum
2.7.11.7 Mg2+ requirement Dictyostelium discoideum
2.7.11.7 Mg2+ maximal activity at 6-8 mM Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.7 84000
-
 x * 84000, SDS-PAGE Dictyostelium discoideum
2.7.11.7 94000
-
 x * 94000, phosphorylated enzyme, SDS-PAGE Dictyostelium discoideum
2.7.11.7 240000
-
 gel filtration Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin I heavy chain Dictyostelium discoideum involved in regulation of myosin II filament assembly ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain Dictyostelium discoideum increased activity during chemotaxis ADP + myosin I heavy chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Dictyostelium discoideum
-
 two heavy chain kinases: one in growth-phase cells and one in starved, developing cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.7 solubilized by high-salt extraction, affinity chromatography, 4600fold, to homogeneity Dictyostelium discoideum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.7 cell culture starved, developing cells Dictyostelium discoideum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.7 2.1
-
 purified enzyme Dictyostelium discoideum

Storage Stability

EC Number Storage Stability Organism
2.7.11.7 -20°C, in 25 mM HEPES buffer, pH 7.5, 1 mM DTT, 1 mM EDTA, 50 mM NaCl, 10% glycerol, or 20% sucrose, stable for 3 months Dictyostelium discoideum
2.7.11.7 -20°C, in 50% sucrose or glycerol, t1/2: 2 weeks Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + casein no activity Dictyostelium discoideum ADP + casein phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase intramolecular autophosphorylation Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase 4 phosphorylation sites on each heavy chain Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase leads to activation of the phosphorylation activity towards myosin I Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain myosin light chains are no substrates Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain 4 mol phosphate per mol of myosin Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain phosphorylates threonine residues Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain 20 mol phosphate per mol of kinase subunit, kinetics Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain bovine muscle myosin is no substrate Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain involved in regulation of myosin II filament assembly Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain increased activity during chemotaxis Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + peptide LMM58 of heavy chain 4 mol phosphate per mol, Thr-residues are phosphorylated Dictyostelium discoideum ?
-
 ?
2.7.11.7 ATP + [myosin heavy-chain]
-
 Dictyostelium discoideum ADP + [myosin heavy-chain] phosphate
-
 ?
2.7.11.7 ATP + [protein LMM58 heavy chain]
-
 Dictyostelium discoideum ADP + [protein LMM58 heavy chain] phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.7 ? x * 84000, SDS-PAGE Dictyostelium discoideum
2.7.11.7 ? x * 94000, phosphorylated enzyme, SDS-PAGE Dictyostelium discoideum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 22
-
 assay at Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 7.5
-
-
 Dictyostelium discoideum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Dictyostelium discoideum