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Literature summary extracted from

  • Nalaskowski, M.M.; Bertsch, U.; Fanick, W.; Stockebrand, M.C.; Schmale, H.; Mayr, G.W.
    Rat inositol 1,4,5-trisphosphate 3-kinase C is enzymatically specialized for basal cellular inositol trisphosphate phosphorylation and shuttles actively between nucleus and cytoplasm (2003), J. Biol. Chem., 278, 19765-19776.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.127 1D-myo-inositol 1,3,4,5-tetrakisphosphate recombinant, catalytically active fragment of isoform C, allosteric product activation in the absence of Ca2+/calmodulin, which per se activates enzyme and abolishes the allosteric effect Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.127 cloning of a full-length cDNA encoding IP3K isoform C from tongue epithelium, cloning of an enzymatically active and Ca2+/calmodulin-regulated fragment of isoform C and expression in Escherichia coli BL21(DE3)pRIL Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.127 1D-myo-inositol 1,3,4,5-tetrakisphosphate marked product inhibition, isoforms A and B Rattus norvegicus
2.7.1.127 1D-myo-inositol 1,4,5-trisphosphate recombinant, catalytically active fragment of isoform C, substrate inhibition by high concentrations Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.127 0.0002
-
 1D-myo-inositol 1,4,5-trisphosphate pH 7.5, 30°C, recombinant, catalytically active fragment of isoform C Rattus norvegicus
2.7.1.127 0.0015
-
 1D-myo-inositol 2,4,5-trisphosphate pH 7.5, 30°C, in absence of Ca2+/calmodulin, recombinant, catalytically active fragment of isoform C Rattus norvegicus
2.7.1.127 0.005
-
 1D-myo-inositol 2,4,5-trisphosphate pH 7.5, 30°C, in presence of 100 nM Ca2+/calmodulin, recombinant, catalytically active fragment of isoform C Rattus norvegicus
2.7.1.127 0.033
-
 ATP pH 7.5, 30°C, in absence of Ca2+/calmodulin, recombinant, catalytically active fragment of isoform C Rattus norvegicus
2.7.1.127 0.052
-
 ATP pH 7.5, 30°C, in presence of 100 nM Ca2+/calmodulin, recombinant, catalytically active fragment of isoform C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.127 cytoplasm isoform C undergoes active nucleocytoplasmic shuttling Rattus norvegicus 5737
-
2.7.1.127 nucleus isoform C is actively transported into and out of the nucleus, a leucine-rich nuclear export signal and a nuclear import activity are localized in the N-terminal domain Rattus norvegicus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.127 Ca2+ isoform C is strongly activated by Ca2+/calmodulin in a substrate concentration-dependent manner Rattus norvegicus
2.7.1.127 Ca2+ activation by Ca2+/calmodulin Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.127 74500
-
 x * 74500, calculated from the amino acid sequence Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus inositol 1,4,5-trisphosphate is a second messenger ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus IP3K isoform C: nucleocytoplasmic shuttling, particular role in nuclear inositol trisphosphate phosphorylation and cellular growth, enzyme seems to function during the intervalls between signaling events, keeps the resting Ins(1,4,5)P3 very low and provides a basal Ins(1,3,4,5)P4 production in the absence of stimulatory signals from the exterior ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus inositol phosphate metabolism ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.127 Rattus norvegicus
-
 isoform C
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.127 brain isoform C Rattus norvegicus
-
2.7.1.127 heart isoform C Rattus norvegicus
-
2.7.1.127 kidney isoform C, low expression Rattus norvegicus
-
2.7.1.127 liver isoform C, low expression Rattus norvegicus
-
2.7.1.127 lung isoform C, low expression Rattus norvegicus
-
2.7.1.127 testis isoform C Rattus norvegicus
-
2.7.1.127 tongue strongest expression of isoform C in tongue epithelium Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate domain structure of IP3K-C Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate specific for phosphorylation of the 3-position Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol 1,4,5-trisphosphate is a second messenger Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate IP3K isoform C: nucleocytoplasmic shuttling, particular role in nuclear inositol trisphosphate phosphorylation and cellular growth, enzyme seems to function during the intervalls between signaling events, keeps the resting Ins(1,4,5)P3 very low and provides a basal Ins(1,3,4,5)P4 production in the absence of stimulatory signals from the exterior Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol phosphate metabolism Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 2,4,5-trisphosphate recombinant, catalytically active fragment of isoform C, in the presence of Ins(1,3,4,5)P4, authentic side activity of isoform C Rattus norvegicus ADP + 1D-myo-inositol 2,4,5,6-tetrakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.127 ? x * 74500, calculated from the amino acid sequence Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.127 30
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.127 7.5
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.127 ATP
-
 Rattus norvegicus
2.7.1.127 Calmodulin activation by Ca2+/calmodulin Rattus norvegicus
2.7.1.127 Calmodulin isoform C is strongly activated by Ca2+/calmodulin in a substrate concentration-dependent manner Rattus norvegicus