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Literature summary extracted from

  • Woodring, P.J.; Garrison, J.C.
    Expression, purification, and regulation of two isoforms of the inositol 1,4,5-trisphosphate 3-kinase (1997), J. Biol. Chem., 272, 30447-30454.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.127 protein kinase A isoforms A and B are differentially regulated via phosphorylation by the cAMP-dependent protein kinase, isoform A: phosphorylated to the extent of 0.9 mol/mol, 2.5fold stimulation in the absence of Ca2+/calmodulin, 1.5fold stimulation in the presence of Ca2+/calmodulin, isoform B: phosphorylated to the extent of 1 mol/mol, no effect on activity in the absence of Ca2+/calmodulin, 45% inhibition in the presence of Ca2+/calmodulin Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.127 cDNAs encoding enzyme isoforms A from brain and B are cloned and overexpressed in B31 cells, a rat-1 fibroblast cell line expressing pp60v-src Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.1.127 2-mercaptoethanol and Triton X-100 or CHAPS stabilize during purification Rattus norvegicus
2.7.1.127 bovine serum albumin stabilizes dilute enzyme solutions Rattus norvegicus
2.7.1.127 isoform A is more susceptible to proteolysis during purification than isoform B Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.127 Ca2+ isoforms A and B, above 0.01 mM Rattus norvegicus
2.7.1.127 protein kinase A isoforms A and B are differentially regulated via phosphorylation by the cAMP-dependent protein kinase, isoform A: stimulation in the presence or absence of Ca2+/calmodulin, isoform B: no effect on activity in the absence of Ca2+/calmodulin, 45% inhibition in the presence of Ca2+/calmodulin Rattus norvegicus
2.7.1.127 Protein kinase C isoforms A and B are differentially regulated via phosphorylation by protein kinase C, isoform A: phosphorylated to the extent of 2 mol/mol, 72% inhibition in the absence of Ca2+/calmodulin, isoform B: phosphorylated to the extent of 2.7 mol/mol, no effect on activity in the absence of Ca2+/calmodulin, both isoforms are inhibited by 70% in the presence of Ca2+/calmodulin Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.127 0.002 0.005 1D-myo-inositol 1,4,5-trisphosphate pH 7.5, 37°C, recombinant isoenzymes A and B Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.127 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.127 Ca2+ isoforms A and B are differentially regulated by Ca2+/calmodulin, isoform A: 2.5fold stimulation, isoform B: 20fold stimulation Rattus norvegicus
2.7.1.127 Ca2+ activation by Ca2+/calmodulin Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.127 59000
-
 x * 59000, recombinant isoform A, x * 92000, recombinant isoform B, SDS-PAGE Rattus norvegicus
2.7.1.127 92000
-
 x * 59000, recombinant isoform A, x * 92000, recombinant isoform B, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus differential expression and regulation of the two enzyme isoforms A and B provides multiple mechanisms for regulating the cytosolic level of inositol 1,4,5-trisphosphate in cells, regulation mechanisms ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus inositol 1,4,5-trisphosphate is a second messenger ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.1.127 Triton X-100 stabilizes Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.127 Rattus norvegicus
-
 2 isoenzymes: A and B
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.127 phosphoprotein isoforms A and B are differentially regulated via phosphorylation by protein kinase C and the cAMP-dependent protein kinase, potential phosphorylation sites Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.127 recombinant isoforms A and B, overexpressed in B31 rat fibroblast, isoform A: 11005fold, isoform B: 43806fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.127 brain isoform A Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.127 15.77
-
 pH 7.5, 37°C, recombinant isoform B Rattus norvegicus
2.7.1.127 36.32
-
 pH 7.5, 37°C, recombinant isoform A Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.127 -70°C, buffer containing detergent and reducing agent, pH 7-8, at least 3 months, stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate multiple regulation mechanisms of enzyme isoform A and B Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate differential expression and regulation of the two enzyme isoforms A and B provides multiple mechanisms for regulating the cytosolic level of inositol 1,4,5-trisphosphate in cells, regulation mechanisms Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol 1,4,5-trisphosphate is a second messenger Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.127 ? x * 59000, recombinant isoform A, x * 92000, recombinant isoform B, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.127 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.127 7.5
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.127 ATP
-
 Rattus norvegicus
2.7.1.127 Calmodulin activation by Ca2+/calmodulin Rattus norvegicus
2.7.1.127 Calmodulin isoforms A and B are differentially regulated by Ca2+/calmodulin, isoform A: 2.5fold stimulation, isoform B: 20fold stimulation Rattus norvegicus