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Literature summary extracted from

  • Communi, D.; Dewaste, V.; Erneux, C.
    Calcium-calmodulin-dependent protein kinase II and protein kinase C-mediated phosphorylation and activation of D-myo-inositol 1,4, 5-trisphosphate 3-kinase B in astrocytes (1999), J. Biol. Chem., 274, 14734-14742.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.127 12-O-tetradecanoylphorbol-13-acetate 2fold activation, isoform B Homo sapiens
2.7.1.127 12-O-tetradecanoylphorbol-13-acetate 2fold activation, isoform B Rattus norvegicus
2.7.1.127 CaM kinase II phosphorylation of isoenzyme B by calmodulin kinase II and protein kinase C added together results in a maximal 60-70fold activation, no effect on the sensitivity to the Ca2+/calmodulin complex, CaM kinase II alone activates 35-40fold in the presence of Ca2+ and calmodulin Homo sapiens
2.7.1.127 CaM kinase II phosphorylation of isoenzyme B by calmodulin kinase II and protein kinase C added together results in a maximal 60-70fold activation, no effect on the sensitivity to the Ca2+/calmodulin complex, CaM kinase II alone activates 35-40fold in the presence of Ca2+ and calmodulin Rattus norvegicus
2.7.1.127 Carbachol calphostin C, KN-93 or KN-62 partially prevents activation Rattus norvegicus
2.7.1.127 Carbachol 6-8fold okadaic acid-sensitive activation, maximal at 0.01 mM, isoform B, carbachol-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction Homo sapiens
2.7.1.127 Carbachol 6-8fold okadaic acid-sensitive activation, maximal at 0.01 mM, isoform B, carbachol-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction Rattus norvegicus
2.7.1.127 additional information isoenzyme B of 1321N1 cells is not activated by UTP Homo sapiens
2.7.1.127 additional information isoenzymes A and B are not activated by protein kinase A Rattus norvegicus
2.7.1.127 Protein kinase C phosphorylation of isoenzyme B by calmodulin kinase II and protein kinase C added together results in a maximal 60-70fold activation, but protein kinase C alone inhibits in the presence of Ca2+ and calmodulin, no effect on the sensitivity to the Ca2+/calmodulin complex Homo sapiens
2.7.1.127 Protein kinase C phosphorylation of isoenzyme B by calmodulin kinase II and protein kinase C added together results in a maximal 60-70fold activation, but protein kinase C alone inhibits in the presence of Ca2+ and calmodulin, no effect on the sensitivity to the Ca2+/calmodulin complex Rattus norvegicus
2.7.1.127 UTP
-
 Homo sapiens
2.7.1.127 UTP 6-8fold okadaic acid-sensitive activation, maximal at 0.01 mM, isoform B, calphostin C, KN-93 or KN-62 partially prevents activation, UTP-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.127 Protein kinase C
-
 Homo sapiens
2.7.1.127 Protein kinase C protein kinase C alone, without CaM kinase II, inhibits in the presence of Ca2+ and calmodulin Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.127 0.0015
-
 1D-myo-inositol 1,4,5-trisphosphate isoform B Homo sapiens
2.7.1.127 0.0015
-
 1D-myo-inositol 1,4,5-trisphosphate isoform B Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.127 particle-bound not activated isoenzyme B: 65% soluble, 35% particulate fraction, carbachol-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction, only 10% remain soluble Homo sapiens
-
-
2.7.1.127 particle-bound not activated isoenzyme B: 65% soluble, 35% particulate fraction, carbachol-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction, only 10% remain soluble Rattus norvegicus
-
-
2.7.1.127 soluble unstimulated isoenzyme B: 65% soluble, 35% particulate fraction, carbachol-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction, only 10% remain soluble Homo sapiens
-
-
2.7.1.127 soluble unstimulated isoenzyme B: 65% soluble, 35% particulate fraction, carbachol-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction, only 10% remain soluble Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.127 Ca2+ phosphorylation of isoenzyme B has no effect on the sensitivity to the Ca2+/calmodulin complex Rattus norvegicus
2.7.1.127 Ca2+ activation by Ca2+/calmodulin Homo sapiens
2.7.1.127 Ca2+ activation by Ca2+/calmodulin Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.127 88000
-
 x * 88000, isoform B, SDS-PAGE, Western blot analysis Homo sapiens
2.7.1.127 88000
-
 x * 88000, isoform B, SDS-PAGE, Western blot analysis Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Homo sapiens regulatory mechanism of isoenzyme B involving phosphorylation by both protein kinase C and CaM kinase II ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus regulatory mechanism of isoenzyme B involving phosphorylation by both protein kinase C and CaM kinase II ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Homo sapiens inositol 1,4,5-trisphosphate is a second messenger ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus inositol 1,4,5-trisphosphate is a second messenger ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.127 Homo sapiens
-
 isoform B
-
2.7.1.127 Rattus norvegicus
-
 2-3 days old, isoform B
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.127 phosphoprotein phosphorylation of enzyme by calmodulin kinase II and protein kinase C added together results in a maximal 60-70fold activation, distinct sites are phosphorylated independently by both protein kinases, prevented by the CaM kinase II inhibitors KN-93, KN-62 and the protein kinase C inhibitor calphostin C Homo sapiens
2.7.1.127 phosphoprotein phosphorylation of enzyme by calmodulin kinase II and protein kinase C added together results in a maximal 60-70fold activation, distinct sites are phosphorylated independently by both protein kinases, prevented by the CaM kinase II inhibitors KN-93, KN-62 and the protein kinase C inhibitor calphostin C Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.127 isoenzyme B Homo sapiens
2.7.1.127 isoenzyme B Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.127 astrocyte cerebral cortex astrocytes, isoform B Rattus norvegicus
-
2.7.1.127 astrocytoma cell 1321N1 cells, isoform B Homo sapiens
-
2.7.1.127 brain
-
 Homo sapiens
-
2.7.1.127 brain cortical astrocytes, isoform B Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.127 1.4
-
 isoform B, in absence of the Ca2+/calmodulin complex Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate regulatory mechanism of isoenzyme B involving phosphorylation by both protein kinase C and CaM kinase II Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate regulatory mechanism of isoenzyme B involving phosphorylation by both protein kinase C and CaM kinase II Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol 1,4,5-trisphosphate is a second messenger Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol 1,4,5-trisphosphate is a second messenger Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.127 ? x * 88000, isoform B, SDS-PAGE, Western blot analysis Homo sapiens
2.7.1.127 ? x * 88000, isoform B, SDS-PAGE, Western blot analysis Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.127 ATP
-
 Homo sapiens
2.7.1.127 ATP
-
 Rattus norvegicus
2.7.1.127 Calmodulin activation by Ca2+/calmodulin Homo sapiens
2.7.1.127 Calmodulin activation by Ca2+/calmodulin Rattus norvegicus
2.7.1.127 Calmodulin phosphorylation of isoenzyme B has no effect on the sensitivity to the Ca2+/calmodulin complex Rattus norvegicus