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Literature summary extracted from

  • Communi, D.; Vanweyenberg, V.; Erneux, C.
    D-myo-inositol 1,4,5-trisphosphate 3-kinase A is activated by receptor activation through a calcium:calmodulin-dependent protein kinase II phosphorylation mechanism (1997), EMBO J., 16, 1943-1952.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.127 CaM kinase II phosphorylates and activates Rattus norvegicus
2.7.1.127 CaM kinase II phosphorylation of Thr-311 results in 8-10fold enzyme activation in the presence of 0.01 mM free Ca2+ and 0.002 mM calmodulin and in a 25fold increase in sensitivity to the Ca2+/calmodulin complex Homo sapiens
2.7.1.127 Carbachol
-
 Homo sapiens
2.7.1.127 Carbachol 3-5fold activation, maximal at 0.01-0.05 mM, isoenzyme A, increases the phosphorylation of enzyme Rattus norvegicus
2.7.1.127 UTP
-
 Rattus norvegicus
2.7.1.127 UTP activation, recombinant isoenzyme A overexpressed in CHO cells Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.127 isoenzyme A is cloned and overexpressed in CHO cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.127 KN-62 calmodulin-dependent protein kinase II inhibitor; prevents the carbachol- or UTP-mediated activation Homo sapiens
2.7.1.127 KN-62 calmodulin-dependent protein kinase II inhibitor; prevents the carbachol- or UTP-mediated activation Rattus norvegicus
2.7.1.127 KN-93 calmodulin-dependent protein kinase II inhibitor; prevents the carbachol- or UTP-mediated activation Homo sapiens
2.7.1.127 KN-93 calmodulin-dependent protein kinase II inhibitor; prevents the carbachol- or UTP-mediated activation Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.127 0.004
-
 1D-myo-inositol 1,4,5-trisphosphate pH 7.4 Homo sapiens
2.7.1.127 0.004
-
 1D-myo-inositol 1,4,5-trisphosphate pH 7.5 Rattus norvegicus
2.7.1.127 0.004
-
 1D-myo-inositol 1,4,5-trisphosphate 37°C, isoform A Homo sapiens
2.7.1.127 0.004
-
 1D-myo-inositol 1,4,5-trisphosphate 37°C, isoform A Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.127 Ca2+ phosphorylation of Thr-311 of isoenzyme A by CaM kinase II results in a 25fold increase in sensitivity to the Ca2+/calmodulin complex Homo sapiens
2.7.1.127 Ca2+ activation by Ca2+/calmodulin Homo sapiens
2.7.1.127 Ca2+ activation by Ca2+/calmodulin Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.127 53000
-
 x * 53000, isoenzyme A, SDS-PAGE Homo sapiens
2.7.1.127 53000
-
 x * 53000, isoenzyme A, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Homo sapiens regulation mechanism of isoenzyme A in brain involving CaM kinase II-mediated phosphorylation, role of Ins(1,4,5)P3 3-kinase in brain ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus regulation mechanism of isoenzyme A in brain involving CaM kinase II-mediated phosphorylation, role of Ins(1,4,5)P3 3-kinase in brain ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus inositol phosphate metabolism ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.127 Homo sapiens
-
 recombinant isoenzyme A overexpressed in CHO cells
-
2.7.1.127 Rattus norvegicus
-
 adult
-
2.7.1.127 Rattus norvegicus
-
 male sprague-dawley rats
-
2.7.1.127 Rattus norvegicus
-
 isoenzyme A
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.127 phosphoprotein
-
 Rattus norvegicus
2.7.1.127 phosphoprotein phosphorylation of Thr-311 by CaM kinase II results in 8-10fold enzyme activation in the presence of 0.01 mM free Ca2+ and 0.002 mM calmodulin and in a 25fold increase in sensitivity to the Ca2+/calmodulin complex Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.127 recombinant isoenzyme A overexpressed in CHO cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.127 brain cortex Rattus norvegicus
-
2.7.1.127 brain isoenzyme A is the major isoform present in neuronal cells Homo sapiens
-
2.7.1.127 brain isoenzyme A is the major isoform present in neuronal cells Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.127 0.0048
-
 37°C, isoenzyme A, rat cortical slices Homo sapiens
2.7.1.127 1.7
-
 pH 7.4, 37°C, purified recombinant isoenzyme A overexpressed in CHO cells Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate regulation mechanism of isoenzyme A involving CaM kinase II-mediated phosphorylation Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate regulation mechanism of isoenzyme A involving CaM kinase II-mediated phosphorylation Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate regulation mechanism of isoenzyme A in brain involving CaM kinase II-mediated phosphorylation, role of Ins(1,4,5)P3 3-kinase in brain Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate regulation mechanism of isoenzyme A in brain involving CaM kinase II-mediated phosphorylation, role of Ins(1,4,5)P3 3-kinase in brain Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol phosphate metabolism Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.127 ? x * 53000, isoenzyme A, SDS-PAGE Homo sapiens
2.7.1.127 ? x * 53000, isoenzyme A, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.127 37
-
 assay at Homo sapiens
2.7.1.127 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.127 7.4
-
 assay at Homo sapiens
2.7.1.127 7.5
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.127 ATP
-
 Homo sapiens
2.7.1.127 ATP
-
 Rattus norvegicus
2.7.1.127 Calmodulin activation by Ca2+/calmodulin Homo sapiens
2.7.1.127 Calmodulin activation by Ca2+/calmodulin Rattus norvegicus
2.7.1.127 Calmodulin phosphorylation of Thr-311 of isoenzyme A by CaM kinase II results in a 25fold increase in sensitivity to the Ca2+/calmodulin complex, maximally 2-2.5fold stimulation by the Ca2+/calmodulin complex Homo sapiens