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Literature summary extracted from

  • Shin, Y.S.; Choi, G.; Choi, K.Y.
    Overexpression, purification and characterization of inositol 1,4,5-triphosphate 3-kinase from rat brain (1995), Mol. Cells, 5, 348-353.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.127 entire gene encoding IP3K is cloned and 130fold overexpressed in Escherichia coli BL21(DE3), when induced by isopropyl-beta-D-thiogalactoside Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.127 0.0021
-
 1D-myo-inositol 1,4,5-trisphosphate recombinant IP3K, in absence of Ca2+/calmodulin, 37°C Rattus norvegicus
2.7.1.127 0.0876
-
 ATP 37°C, recombinant IP3K Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.127 cytosol mainly Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.127 Ca2+ 1.5fold activation by Ca2+/calmodulin Rattus norvegicus
2.7.1.127 Ca2+ activation by Ca2+/calmodulin Rattus norvegicus
2.7.1.127 Ca2+ not activated by free Ca2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.127 50000
-
 x * 50000, recombinant IP3K overexpressed in Escherichia coli, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus IP3K regulates the intracellular level of the 2 second messengers Ins(1,4,5)P3 and Ins(1,3,4,5)P4 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus inositol phosphate metabolism ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.127 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.127 83fold Rattus norvegicus
2.7.1.127 recombinant IP3K overexpressed in Escherichia coli Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.127 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.127 20
-
 recombinant IP3K overexpressed in Escherichia coli, in absence of calmodulin Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate IP3K regulates the intracellular level of the 2 second messengers Ins(1,4,5)P3 and Ins(1,3,4,5)P4 Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol phosphate metabolism Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.127 ? x * 50000, recombinant IP3K overexpressed in Escherichia coli, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.127 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.127 7.5
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.127 ATP
-
 Rattus norvegicus
2.7.1.127 Calmodulin activation by Ca2+/calmodulin Rattus norvegicus
2.7.1.127 Calmodulin 1.5fold activation by Ca2+/calmodulin Rattus norvegicus