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Literature summary extracted from

  • Lee, S.Y.; Sim, S.S.; Kim, J.W.; Moon, K.H.; Kim, J.H.; Rhee, S.G.
    Purification and properties of D-myo-inositol 1,4,5-trisphosphate 3-kinase from rat brain. Susceptibility to calpain (1990), J. Biol. Chem., 265, 9434-9440.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.127 0.1% CHAPS or 0.1% Triton X-100 stabilizes, 0.5 mg/ml bovine serum albumin is less efficient in stabilizing Rattus norvegicus
2.7.1.127 bovine serum albumin stabilizes dilute enzyme solutions Rattus norvegicus
2.7.1.127 calpain inhibitors stabilize during purification Rattus norvegicus
2.7.1.127 ethylene glycol, 10%, KCl or sucrose, 0.25 M, does not stabilize Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.127 additional information enzyme contains several motifs susceptible to a variety of proteases Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.127 Ca2+ activation by Ca2+/calmodulin Rattus norvegicus
2.7.1.127 Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.127 53000
-
 1 * 53000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate Rattus norvegicus involved in inositol polyphosphate metabolism ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.1.127 Triton X-100 0.1%, stabilizes Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.127 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.127 16100fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.127 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.127 28.9
-
 pH 7.5, 37°C, in absence of calmodulin Rattus norvegicus
2.7.1.127 60
-
 pH 7.5, 37°C, in presence of calmodulin Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.127 -20°C or 4°C, 0.1% CHAPS or Triton X-100, 4 days, less than 5% loss of activity Rattus norvegicus
2.7.1.127 -20°C, 4 days, 58% loss of activity Rattus norvegicus
2.7.1.127 4°C, 4 days, 20% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate involved in inositol polyphosphate metabolism Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.127 monomer 1 * 53000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.127 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.127 7.5
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.127 ATP
-
 Rattus norvegicus
2.7.1.127 Calmodulin activation by Ca2+/calmodulin Rattus norvegicus