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Literature summary extracted from

  • Kikkawa, S.; Yoshida, N.; Nakagawa, M.; Iwasa, T.; Tsuda, M.
    A novel rhodopsin kinase in Octopus photoreceptor possesses a pleckstrin homology domain and is activated by G protein betagamma-subunits (1998), J. Biol. Chem., 273, 7441-7447.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.14 betagamma-subunit of a photoreceptor G protein directly activates, 2.5fold Enteroctopus dofleini
2.7.11.14 spermine at low concentrations: up to 20% activation, inhibits at higher concentrations Enteroctopus dofleini

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.14 ork gene encoding RK is cloned and sequenced Enteroctopus dofleini

General Stability

EC Number General Stability Organism
2.7.11.14 ethylene glycol is used as stabilizer Enteroctopus dofleini

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.14 Dextran sulfate strong Enteroctopus dofleini
2.7.11.14 heparin potent inhibitor Enteroctopus dofleini
2.7.11.14 mastoparan potent inhibitor Enteroctopus dofleini
2.7.11.14 polyanion e.g. heparin, dextran sulfate, polyglutamic acid Enteroctopus dofleini
2.7.11.14 polycation e.g. polyamines or polylysine, potent inhibitor Enteroctopus dofleini
2.7.11.14 Polyglutamic acid weak Enteroctopus dofleini
2.7.11.14 polylysine potent inhibitor Enteroctopus dofleini
2.7.11.14 spermidine potent inhibitor Enteroctopus dofleini
2.7.11.14 spermine at higher concentrations, activates at low concentrations Enteroctopus dofleini

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.14 membrane photoreceptor microvillar membranes, in both the soluble and membrane fractions Enteroctopus dofleini 16020
-
2.7.11.14 soluble in both the soluble and membrane fractions Enteroctopus dofleini
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+
-
 Enteroctopus dofleini

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.14 additional information
-
 amino acid sequence shows high degree of homology to beta-adrenergic receptor kinase primary structure Enteroctopus dofleini
2.7.11.14 80000
-
 x * 80000, SDS-PAGE, predicted from the ork gene sequence Enteroctopus dofleini

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Enteroctopus dofleini
-
 ADP + phosphorhodopsin
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.11.14 Ethylene glycol used as stabilizer Enteroctopus dofleini

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Enteroctopus dofleini O97020
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.14 additional information not isoprenylated Enteroctopus dofleini

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14 17.2fold Enteroctopus dofleini

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Enteroctopus dofleini
-
2.7.11.14 additional information not in brain, optic lobe, testis, liver, muscle, salivary gland, skin Enteroctopus dofleini
-
2.7.11.14 retina photoreceptor microvillar membranes Enteroctopus dofleini
-
2.7.11.14 retina retina-specific expression Enteroctopus dofleini
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.14 0.046
-
 pH 7.4, 15°C Enteroctopus dofleini

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + rhodopsin
-
 Enteroctopus dofleini ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin substrates: octopus rhodopsin, rhodopsin-containing phospholipid vesicles Enteroctopus dofleini ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin light-dependent phosphorylation Enteroctopus dofleini ADP + phosphorhodopsin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.14 ? x * 80000, SDS-PAGE, predicted from the ork gene sequence Enteroctopus dofleini

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 15
-
 assay at Enteroctopus dofleini

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 7.4
-
-
 Enteroctopus dofleini