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Literature summary extracted from

  • Cha, K.; Bruel, C.; Inglese, J.; Khorana, H.G.
    Rhodopsin kinase: Expression in baculovirus-infected insect cells, and characterization of post-translational modifications (1997), Proc. Natl. Acad. Sci. USA, 94, 10577-10582.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.14 RK gene expression in baculovirus-infected Sf21 cells Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+
-
Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.14 62000
-
x * 62000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus
-
ADP + phosphorhodopsin
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.14 phosphoprotein enzyme is autophosphorylated Bos taurus
2.7.11.14 phosphoprotein native RK: mixture of mono- and diphosphorylated forms, two main fractions of purified recombinant RK expressed in Sf21 cells differ in their phosphorylation state: one is monophosphorylated, the other is diphosphorylated Bos taurus
2.7.11.14 side-chain modification native RK is isoprenylated, purified recombinant RK expressed in Sf21 cells: isoprenyl groups consist of mixtures of C5, C10, C15 and C20 isoprenyl moieties, no specific isoprenylation Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14 native and recombinant RK purified from baculovirus-infected Sf21 cells: 63fold Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 retina rod cell outer segment Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.14 0.097
-
pH 7.5, 30°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + rhodopsin
-
Bos taurus ADP + phosphorhodopsin
-
?

Subunits

EC Number Subunits Comment Organism
2.7.11.14 ? x * 62000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 30
-
assay at Bos taurus
2.7.11.14 36
-
about, native RK and recombinant RK expressed in Sf21 cells Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 6.5
-
recombinant RK expressed in Sf21 cells Bos taurus
2.7.11.14 7
-
native RK Bos taurus
2.7.11.14 7.5
-
assay at Bos taurus