EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.11.14 | RK gene expression in baculovirus-infected Sf21 cells | Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.14 | Mg2+ | - |
Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.11.14 | 62000 | - |
x * 62000, SDS-PAGE | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.14 | ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.11.14 | Bos taurus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.11.14 | phosphoprotein | enzyme is autophosphorylated | Bos taurus |
2.7.11.14 | phosphoprotein | native RK: mixture of mono- and diphosphorylated forms, two main fractions of purified recombinant RK expressed in Sf21 cells differ in their phosphorylation state: one is monophosphorylated, the other is diphosphorylated | Bos taurus |
2.7.11.14 | side-chain modification | native RK is isoprenylated, purified recombinant RK expressed in Sf21 cells: isoprenyl groups consist of mixtures of C5, C10, C15 and C20 isoprenyl moieties, no specific isoprenylation | Bos taurus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.11.14 | native and recombinant RK purified from baculovirus-infected Sf21 cells: 63fold | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.11.14 | eye | retina | Bos taurus | - |
2.7.11.14 | retina | rod cell outer segment | Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.11.14 | 0.097 | - |
pH 7.5, 30°C | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.14 | ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.11.14 | ? | x * 62000, SDS-PAGE | Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.11.14 | 30 | - |
assay at | Bos taurus |
2.7.11.14 | 36 | - |
about, native RK and recombinant RK expressed in Sf21 cells | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.11.14 | 6.5 | - |
recombinant RK expressed in Sf21 cells | Bos taurus |
2.7.11.14 | 7 | - |
native RK | Bos taurus |
2.7.11.14 | 7.5 | - |
assay at | Bos taurus |