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Literature summary extracted from

  • Sokal, I.; Pulvermüller, A.; Buczylko, J.; Hofmann, K.P.; Palczewski, K.
    Rhodopsin and its kinase (2001), Methods Enzymol., 343, 578-600.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.14 polycation activates approximately 2fold Bos taurus

Application

EC Number Application Comment Organism
2.7.11.14 medicine mutation in RK is associated with the Oguchi disease Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.14 RK gene is located on chromosome 13q34 Homo sapiens
2.7.11.14 RK is cloned and expressed in SF9 cells Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.14 chelator
-
 Bos taurus
2.7.11.14 Mg2+ at high concentrations; requirement at lower concentrations Bos taurus
2.7.11.14 polyanion
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+
-
 Homo sapiens
2.7.11.14 Mg2+
-
 Rattus norvegicus
2.7.11.14 Mg2+ requirement Bos taurus
2.7.11.14 Mg2+ 1 mM required Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Rattus norvegicus
-
 ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin Homo sapiens important enzyme of phototransduction ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin Bos taurus involved in a mechanism for quenching or terminating the visual signal involving the interaction of metarhodopsin II with RK and arrestin, phosphorylation of light-activated rhodopsin by RK is the key step in the signal-termination reaction ADP + phosphorhodopsin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-
2.7.11.14 Homo sapiens
-
-
-
2.7.11.14 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.14 phosphoprotein autophosphorylation of RK causes a lower affinity of enzyme for photolyzed rhodopsin allowing dissociation Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14
-
 Bos taurus
2.7.11.14 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Homo sapiens
-
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 pineal gland
-
 Rattus norvegicus
-
2.7.11.14 pineal gland
-
 Bos taurus
-
2.7.11.14 retina
-
 Homo sapiens
-
2.7.11.14 retina rod cell outer segment Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.14 0.1 0.7
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + peptide acid-rich peptides, RK prefers acid residues localized to the C-terminal side of the serine Bos taurus ADP + phosphopeptide
-
 ?
2.7.11.14 ATP + peptide containing sites phosphorylated in rhodopsin Bos taurus ADP + phosphopeptide
-
 ?
2.7.11.14 ATP + rhodopsin
-
 Rattus norvegicus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin preferred substrate: ATP Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin phosphorylates bovine rhodopsin Homo sapiens ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin phosphorylates bovine rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin highly specific for photobleached rhodopsin Homo sapiens ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin highly specific for photobleached rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin RK binds to the cytoplasmic loops of photolyzed rhodopsin, forming a stable complex, and then phosphorylating it at the C-terminus, phosphorylation at different sites, including Ser-334, Ser-338 and Ser342, may play different roles in phototransduction Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin domain structure Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin important enzyme of phototransduction Homo sapiens ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin involved in a mechanism for quenching or terminating the visual signal involving the interaction of metarhodopsin II with RK and arrestin, phosphorylation of light-activated rhodopsin by RK is the key step in the signal-termination reaction Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 GTP + rhodopsin very poor substrate Bos taurus GDP + phosphorhodopsin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 30
-
 assay at Homo sapiens
2.7.11.14 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 6 8
-
 Bos taurus
2.7.11.14 7.5
-
-
 Homo sapiens
2.7.11.14 7.5
-
 assay at, photolyzed rhodopsin substrate Bos taurus
2.7.11.14 8.5
-
 assay at, synthetic peptide substrates Bos taurus