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Literature summary extracted from

  • Senin, I.I.; Koch, K.W.; Akhtar, M.; Philippov, P.P.
    Ca2+-dependent control of rhodopsin phosphorylation: Recoverin and rhodopsin kinase (2002), Adv. Exp. Med. Biol., 514, 69-99.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.14 rhodopsin RK normally exists in an inactive resting state and is only activated following interaction with photoactivated rhodopsin Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.14 cDNA encoding RK is characterized and sequenced, 561 amino acids protein Bos taurus
2.7.11.14 RK gene encodes a 564 amino acids polypeptide Homo sapiens
2.7.11.14 RK gene is located on chromosome 13q34 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.14 S488A autophosphorylation site mutant with increased activity for the phosphorylation of rhodopsin in the dark Bos taurus
2.7.11.14 S489A autophosphorylation site mutant with increased activity for the phosphorylation of rhodopsin in the dark Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.14 recoverin ATP inhibits and ADP enhances the RK-recoverin interaction, inhibition mechanism; Ca2+-dependent inhibition Bos taurus
2.7.11.14 recoverin Ca2+-dependent inhibition Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+
-
 Mus musculus
2.7.11.14 Mg2+
-
 Homo sapiens
2.7.11.14 Mg2+
-
 Rattus norvegicus
2.7.11.14 Mg2+
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.14 additional information
-
 amino acid sequence shows high degree of homology to beta-adrenergic receptor kinase primary structure Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Homo sapiens
-
 ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin Rattus norvegicus
-
 ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin Bos taurus deactivation of photoexcited rhodopsin by its phosphorylation by RK, in vivo, since ATP is present, RK exists in an autophosphorylated state ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin Mus musculus the deactivation of photoexcited rhodopsin requires multiple phosphorylations ADP + phosphorhodopsin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-
2.7.11.14 Homo sapiens
-
-
-
2.7.11.14 Mus musculus
-
-
-
2.7.11.14 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.14 phosphoprotein incubation of purified RK with ATP leads to its autophosphorylation, resulting in the modification of 3 serine residues in positions 21, 488 and 489, the presence of phosphoryl groups may play a regulatory role Bos taurus
2.7.11.14 side-chain modification
-
 Bos taurus
2.7.11.14 side-chain modification incorporation of a farnesyl moiety at the C-terminal cysteine residue of the mature protein Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Mus musculus
-
2.7.11.14 eye retina Homo sapiens
-
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 retina
-
 Homo sapiens
-
2.7.11.14 retina rod cell outer segment Mus musculus
-
2.7.11.14 retina rod cell outer segment Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.14 additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + peptide
-
 Bos taurus ADP + phosphopeptide
-
 ?
2.7.11.14 ATP + rhodopsin
-
 Homo sapiens ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin
-
 Rattus norvegicus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin light-dependent phosphorylation Mus musculus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin light-dependent phosphorylation Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin phosphorylation sites of bleached rhodopsin, hierarchical order Mus musculus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin preferred substrate: light-activated form of rhodopsin, i.e. metarhodopsin II Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin RK normally exists in an inactive resting state and is only activated following interaction with photoactivated rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin high-gain phosphorylation of rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin deactivation of photoexcited rhodopsin by its phosphorylation by RK, in vivo, since ATP is present, RK exists in an autophosphorylated state Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin the deactivation of photoexcited rhodopsin requires multiple phosphorylations Mus musculus ADP + phosphorhodopsin
-
 ?