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Literature summary extracted from

  • Dean, K.R.; Akhtar, M.
    Novel mechanism for the activation of rhodopsin kinase: Implications for other G protein-coupled receptor kinases (GRK's) (1996), Biochemistry, 35, 6164-6172.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.14 rhodopsin RK normally exists in an inactive resting state and is only activated following interaction with photoactivated rhodopsin Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.14 membrane rod membranes Bos taurus 16020
-
2.7.11.14 soluble soluble active kinase Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.14 65000
-
 x * 65000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus
-
 ADP + phosphorhodopsin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.14 additional information RK is present predominantly in a nonphosphorylated form Bos taurus
2.7.11.14 phosphoprotein enzyme is autophosphorylated Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 retina rod cell outer segment Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.14 0.18 0.55 32°C Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.11.14 0°C, 20 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane, 2 mM MgCl2, 0.1 mM EDTA, 1 mM dithiothreitol, pH 7.4, 280 mM NaCl, 0.004% Tween 80, 1 mM benzamidine, 0.1 mM phenylmethanesulfonylfluoride, 5 days, 10% loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + 338-SKTETSQVAPA-348 peptide containing the last 11 amino acids of the C-terminal of bovine rhodopsin Bos taurus ?
-
 ?
2.7.11.14 ATP + 338-SKTETSQVAPA-348 phosphorylated at Ser-343, about 11% of the rate with rhodopsin, photoactivated rhodopsin-dependent, soluble active kinase catalyzes photoactivated rhodopsin-independent peptide phosphorylation Bos taurus ?
-
 ?
2.7.11.14 ATP + rhodopsin
-
 Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin the interaction of RK-ATP complex with photoactivated rhodopsin leads to the formation, presumably due to the reorganization of the protein structure, of a soluble active kinase species which reverts to the inactive resting state in a time-dependent fashion, the active kinase catalyzes a photoactivated rhodopsin-independent peptide phosphorylation and dark-phosphorylation of rhodopsin, two-step model for enzyme activation and catalysis Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin incorporation of 1.8 mol phosphate/mol of RK Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin phosphorylates bovine rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin preferred substrate: light-activated form of rhodopsin, i.e. metarhodopsin II Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin RK normally exists in an inactive resting state and is only activated following interaction with photoactivated rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin high-gain phosphorylation of rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.14 ? x * 65000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 32
-
 assay at Bos taurus