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Literature summary extracted from

  • Yu, Q.M.; Cheng, Z.J.; Zhao, J.; Zhou, T.H.; Wu, Y.L.; Ma, L.; Pei, G.
    Carboxyl terminal of rhodopsin kinase is required for the phosphorylation of photo-activated rhodopsin (1998), Cell Res., 8, 303-310.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.11.14 medicine mutations occuring in the variable region of RK C-terminus and catalytic domain cause Oguchi disease, an inherited form of stationary night blindness Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.14 cDNAs encoding full-length RK and C-terminus-truncated mutant RK lacking the last 59 amino acids are cloned and expressed in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.14 additional information 50 kDa C-terminus-truncated mutant RK lacking the last 59 amino acids shows abolished light-dependent translocation and is unable to phosphorylate photoactivated rhodopsin, but phosphorylates the small peptide substrate RRREEEEESAAA like wild-type RK Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.14 0.004 0.0044 rhodopsin pH 7.5, 30°C Homo sapiens
2.7.11.14 2
-
RRREEEEESAAA pH 7.5, 30°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.14 cytosol recombinant RK, in both plasma membranes and cytosolic fractions Homo sapiens 5829
-
2.7.11.14 plasma membrane recombinant RK, in both plasma membranes and cytosolic fractions Homo sapiens 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+
-
Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.14 63000
-
x * 63000, Western blot analysis Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Homo sapiens natural substrate is photoactivated rhodopsin ADP + phosphorhodopsin
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + rhodopsin C-terminus is required for phosphorylation of photo-activated rhodopsin and may be involved in interaction with it Homo sapiens ADP + phosphorhodopsin
-
?
2.7.11.14 ATP + rhodopsin specific for photoactivated rhodopsin, time-dependent phosphorylation, light-dependent translocation, i.e. association of the kinase with photoactivated rhodopsin Homo sapiens ADP + phosphorhodopsin
-
?
2.7.11.14 ATP + rhodopsin natural substrate is photoactivated rhodopsin Homo sapiens ADP + phosphorhodopsin
-
?
2.7.11.14 ATP + RRREEEEESAAA synthetic peptide substrate Homo sapiens ADP + RRREEEEE-(P)SAAA
-
?

Subunits

EC Number Subunits Comment Organism
2.7.11.14 ? x * 63000, Western blot analysis Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 30
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 7.5
-
assay at Homo sapiens